Human/Mouse/Rat Keap1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3024G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3024G-100UG

Save 15% on Select RUO Reagents. See Details.

All Keap1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Keap1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat Keap1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Keap1
Ala90-Ile250
Accession # Q14145

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Keap1

Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.

Long Name

Kelch-like ECH-associated Protein 1

Entrez Gene IDs

9817 (Human)

Alternate Names

INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887