Home / MLK4 alpha / Human/Mouse/Rat MLK4 alpha Alexa Fluor® 647-conjugated Antibody

Human/Mouse/Rat MLK4 alpha Alexa Fluor® 647-conjugated Antibody

Catalog #: AF3435R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3435R-100UG

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Human/Mouse/Rat MLK4 alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat MLK4 alpha. Because the peptide immunogen corresponds to a region at the C-terminus of MLK4 alpha absent in MLK4 beta, no reactivity with MLK4 beta is present.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Human MLK4α synthetic peptide (aa 560 - 570)
ELSALPPGLLC
Accession # CAC84639
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MLK4 alpha

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the p38 and JNK signaling cascades. The potential importance of MLK4’s role in cell regulation has been indicated by the finding of nine separate mlk4 point mutations in a recent sampling of colorectal cancers. MLK4 has two splice variants, with MLK4 alpha containing a different and shorter C-terminus than MLK4 beta.

Long Name
Mixed Lineage Kinase 4 alpha
Entrez Gene IDs
84451 (Human)
Alternate Names
dJ862P8.3; KIAA1804; MAP3K21; MLK4 alpha; MLK4

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