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Human/Mouse/Rat PKN2 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB5686X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5686X-100UG

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Human/Mouse/Rat PKN2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PKN2 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 509105
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human PKN2
Asp18-Asn207
Accession # Q16513
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKN2

Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.

Long Name
Protein Kinase N2
Entrez Gene IDs
5586 (Human); 109333 (Mouse); 207122 (Rat)
Alternate Names
cardiolipin-activated protein kinase Pak2; EC 2.7.11; EC 2.7.11.13; PAK2; Pak-2; PKN2; PRK2; PRK2MGC150606; PRKCL2; PRKCL2PRO2042; PRO2042; Protein kinase C-like 2MGC71074; protein kinase N2; Protein-kinase C-related kinase 2; serine/threonine-protein kinase N2

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