Detection of Human/Mouse/Rat PKN2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, TF‑1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKN2 Monoclonal Antibody (Catalog # MAB5686) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.
Protein Kinase N2
Entrez Gene IDs:
5586 (Human); 109333 (Mouse); 207122 (Rat)
cardiolipin-activated protein kinase Pak2; EC 2.7.11; EC 18.104.22.168; PAK2; Pak-2; PRK2; PRK2MGC150606; PRKCL2; PRKCL2PRO2042; PRO2042; Protein kinase C-like 2MGC71074; protein kinase N2; Protein-kinase C-related kinase 2; serine/threonine-protein kinase N2
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