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Human/Mouse/Rat PRL-3 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB3219G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3219G-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PRL-3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.
Source
Monoclonal Mouse IgG2b Clone # 334402
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PRL-3/PTP4A3

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Long Name
Phosphatase of Regenerating Liver 3
Entrez Gene IDs
11156 (Human); 19245 (Mouse)
Alternate Names
PRL3; PRL-3; PRL3EC 3.1.3.48; PRL-3potentially prenylated protein tyrosine phosphatase; PRL-Rprotein tyrosine phosphatase type IVA 3; protein tyrosine phosphatase type IVA, member 3; Protein-tyrosine phosphatase 4a3; Protein-tyrosine phosphatase of regenerating liver 3; PTP4A3

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