Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Zebrafish
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 334402
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Product Specifications
Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Ala2-Met173
Accession # O75365
Specificity
Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat PRL‑3 Antibody
Detection of Human/Mouse/Rat PRL‑3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PRL‑3 Antibody
Application
Recommended Usage
Western Blot
0.3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line
Reviewed Applications
Read 3 reviews rated 3.7 using MAB3219 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PRL-3/PTP4A3
Long Name
Phosphatase of Regenerating Liver 3
Alternate Names
PRL3, PTP4A3
Gene Symbol
PTP4A3
UniProt
Additional PRL-3/PTP4A3 Products
Product Documents for Human/Mouse/Rat PRL‑3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat PRL‑3 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse/Rat PRL‑3 Antibody
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Customer Reviews for Human/Mouse/Rat PRL‑3 Antibody (3)
3.7 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/03/2020
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 02/21/2019
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/11/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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