Human/Mouse/Rat PRL-3 Antibody

Catalog # Availability Size / Price Qty
MAB3219
MAB3219-SP
Human/Mouse/Rat PRL-3 Antibody in Data
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse/Rat PRL-3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.
Source
Monoclonal Mouse IgG2B Clone # 334402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat PRL-3 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PRL‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRL-3/PTP4A3

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Long Name
Phosphatase of Regenerating Liver 3
Entrez Gene IDs
11156 (Human); 19245 (Mouse)
Alternate Names
PRL3; PRL-3; PRL3EC 3.1.3.48; PRL-3potentially prenylated protein tyrosine phosphatase; PRL-Rprotein tyrosine phosphatase type IVA 3; protein tyrosine phosphatase type IVA, member 3; Protein-tyrosine phosphatase 4a3; Protein-tyrosine phosphatase of regenerating liver 3; PTP4A3

Product Datasheets

Citations for Human/Mouse/Rat PRL-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tumor necrosis factor alpha-induced microRNA-18a activates rheumatoid arthritis synovial fibroblasts through a feedback loop in NF-kappaB signaling.
    Authors: Trenkmann M, Brock M, Gay R, Michel B, Gay S, Huber L
    Arthritis Rheum, 2013;65(4):916-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma.
    Authors: Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M
    Int. J. Cancer, 2010;127(3):543-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A selective phosphatase of regenerating liver phosphatase inhibitor suppresses tumor cell anchorage-independent growth by a novel mechanism involving p130Cas cleavage.
    Authors: Daouti S, Li WH, Qian H, Huang KS, Holmgren J, Levin W, Reik L, McGady DL, Gillespie P, Perrotta A, Bian H, Reidhaar-Olson JF, Bliss SA, Olivier AR, Sergi JA, Fry D, Danho W, Ritland S, Fotouhi N, Heimbrook D, Niu H
    Cancer Res., 2008;68(4):1162-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Expression and prognostic impact of PRL-3 in lymph node metastasis of gastric cancer: its molecular mechanism was investigated using artificial microRNA interference.
    Authors: Wang Z, He YL, Cai SR, Zhan WH, Li ZR, Zhu BH, Chen CQ, Ma JP, Chen ZX, Li W, Zhang LJ
    Int. J. Cancer, 2008;123(6):1439-47.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat PRL-3 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human/Mouse/Rat PRL-3 Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat PRL-3 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat PRL-3 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human