Human/Mouse/Rat Prohibitin 2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4794N Datasheet / COA / SDS

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AF4794N-100UG

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Human/Mouse/Rat Prohibitin 2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Prohibitin 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Prohibitin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Prohibitin 2
Lys89-Lys299
Accession # Q99623

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Prohibitin 2

Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37 kDa, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1‑50), two ER repressor domains (aa 19‑49 and aa 150‑174), and a central coiled-coil domain (aa 190‑238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89‑299, mouse and rat PHB2 are 100% identical to human PHB2.

Entrez Gene IDs

11331 (Human); 12034 (Mouse); 114766 (Rat)

Alternate Names

BAP; Bap37; BCAP37; B-cell associated protein; D-Prohibitin; MGC117268; p22; PHB2; PNAS-141; Prohibitin 2; prohibitin-2; REA; REAB-cell receptor-associated protein BAP37; Repressor of estrogen receptor activity