Human/Mouse/Rat Prohibitin 2 Antibody

Catalog #: AF4794 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4794
AF4794-SP

Detection of Human Prohibitin 2 by Western Blot.

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All Prohibitin 2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Prohibitin 2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Prohibitin 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Prohibitin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Prohibitin 2
Lys89-Lys299
Accession # Q99623

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Prohibitin 2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Prohibitin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4794) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prohibitin 2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prohibitin 2

Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37 kDa, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1‑50), two ER repressor domains (aa 19‑49 and aa 150‑174), and a central coiled-coil domain (aa 190‑238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89‑299, mouse and rat PHB2 are 100% identical to human PHB2.

Entrez Gene IDs

11331 (Human); 12034 (Mouse); 114766 (Rat)

Alternate Names

BAP; Bap37; BCAP37; B-cell associated protein; D-Prohibitin; MGC117268; p22; PHB2; PNAS-141; Prohibitin 2; prohibitin-2; REA; REAB-cell receptor-associated protein BAP37; Repressor of estrogen receptor activity