Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9125A-025
Detection  of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow  Cytometry.
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Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1181A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli derived recombinant human ROR gamma T/RORC2/NR1F3
Met1-Gln100
Accession # P51449
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection  of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow  Cytometry. View Larger

Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Monoclonal Antibody (Catalog # IC9125A) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection  of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Monoclonal Antibody (Catalog # IC9125A) and Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Entrez Gene IDs
6097 (Human)
Alternate Names
ROR gamma t; RORC2

Product Datasheets

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Isotype Controls

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