Human/Mouse RPTOR Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5957G-100UG
R&D Systems Antibodies
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Citations (1)
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Human/Mouse RPTOR Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse RPTOR in Western blots.
Source
Monoclonal Mouse IgG2b Clone # 514208
Immunogen
E. coli-derived recombinant human RPTOR
Lys77-Gln230
Accession # Q8N122
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RPTOR

RPTOR (Raptor) is a 150 kDa component of the cytosolic mammalian target of Rapamycin complex 1 (mTORC1) which also contains mTOR and GBL proteins. mTORC1 plays a dominant role in cell cycle regulation in response to metabolic conditions. The interaction of RPTOR with the kinase mTOR is stabilized under conditions of nutrient deprivation and energy stress, leading to inhibition of mTOR and cell cycle arrest. RPTOR contains multiple Ser and Thr residues whose phosphorylation regulates the activation status of mTOR. RPTOR is critical for the response of skeletal muscle and adipose tissue to insulin. It contains three RNC blocks (aa 48-511), three HEAT repeats (aa 550-667), and seven C-terminal WD40 domains (aa 1020-1335). Within aa 77-230, human RPTOR shares 100% aa sequence identity with mouse and rat RPTOR.

Long Name
Regulatory Associated Protein of mTOR
Entrez Gene IDs
57521 (Human); 74370 (Mouse); 287871 (Rat)
Alternate Names
KIAA1303; KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; KOG1; Mip1; p150 target of rapamycin (TOR)-scaffold protein; Raptor; regulatory associated protein of MTOR, complex 1; regulatory-associated protein of mTOR; RPTOR

Product Datasheets

Citation for Human/Mouse RPTOR Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function
    Authors: F Wang, M Meng, B Mo, Y Yang, Y Ji, P Huang, W Lai, X Pan, T You, H Luo, X Guan, Y Deng, S Yuan, J Chu, M Namaka, T Hughes, L Ye, J Yu, X Li, Y Deng
    Nat Commun, 2018;9(1):4874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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