Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 514208
View all RPTOR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RPTOR
Lys77-Gln230
Accession # Q8N122

Specificity

Detects human and mouse RPTOR in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse RPTOR Antibody

Detection of Human and Mouse RPTOR antibody by Western Blot.

Detection of Human and Mouse RPTOR by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RPTOR at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of RPTOR antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of RPTOR in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of RPTOR antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of RPTOR in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse RPTOR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line

Reviewed Applications

Read 1 review rated 3 using MAB5957 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RPTOR

RPTOR (Raptor) is a 150 kDa component of the cytosolic mammalian target of Rapamycin complex 1 (mTORC1) which also contains mTOR and GBL proteins. mTORC1 plays a dominant role in cell cycle regulation in response to metabolic conditions. The interaction of RPTOR with the kinase mTOR is stabilized under conditions of nutrient deprivation and energy stress, leading to inhibition of mTOR and cell cycle arrest. RPTOR contains multiple Ser and Thr residues whose phosphorylation regulates the activation status of mTOR. RPTOR is critical for the response of skeletal muscle and adipose tissue to insulin. It contains three RNC blocks (aa 48-511), three HEAT repeats (aa 550-667), and seven C-terminal WD40 domains (aa 1020-1335). Within aa 77-230, human RPTOR shares 100% aa sequence identity with mouse and rat RPTOR.

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Entrez Gene IDs

57521 (Human); 74370 (Mouse); 287871 (Rat)

Gene Symbol

RPTOR

UniProt

Q8N122 (Human)

Additional RPTOR Products

Product Documents for Human/Mouse RPTOR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse RPTOR Antibody

For research use only

Related Research Areas

Autophagy
Interferons

Customer Reviews for Human/Mouse RPTOR Antibody (1)

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  • Human/Mouse RPTOR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue
    Species: Mouse
    Verified Customer | Posted 10/23/2019
    Human/Mouse RPTOR Antibody MAB5957

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