Human/Mouse S1P5/EDG-8 PE-conjugated Antibody

FAB3964P has been discontinued. View all S1P5/EDG-8 products.
    
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse S1P5/EDG‑8 in flow cytometry. Stains human and mouse S1P5/EDG‑8 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 282503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
    Met1-Asp398
    Accession # Q9H228
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG‑8 was stained with Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) and either (A) Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected With Mouse S1P5/EDG‑8 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG‑8 and eGFP was stained with either (A) Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P5/EDG-8
S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.
  • Long Name:
    Endothelial Differentiation Gene 8
  • Entrez Gene IDs:
    53637 (Human); 94226 (Mouse); 60399 (Rat)
  • Alternate Names:
    EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2
Related Research Areas

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