Human/Mouse S1P5/EDG-8 PE-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse S1P5/EDG‑8 in flow cytometry. Stains human and mouse S1P5/EDG‑8 transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 282503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
    Met1-Asp398
    Accession # Q9H228
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG‑8 was stained with Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) and either (A) Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected With Mouse S1P5/EDG‑8 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG‑8 and eGFP was stained with either (A) Mouse Anti-Human S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB3964P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P5/EDG-8
S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.
  • Long Name:
    Endothelial Differentiation Gene 8
  • Entrez Gene IDs:
    53637 (Human); 94226 (Mouse); 60399 (Rat)
  • Alternate Names:
    EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse S1P5/EDG-8 PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information