Human/Mouse SSEA-1 PerCP-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SSEA‑1 in Undifferentiated D3 Mouse Cell Line by Flow Cytometry. Undifferentiated D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2155C, filled histogram) or isotype control antibody (Catalog # IC015C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
- Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
- Fox, N. et al. (1983) Cancer Res. 43:669.
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