Human/Mouse SSEA-1 PerCP-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse SSEA-1.
  • Source
    Monoclonal Mouse IgM Clone # MC-480
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    F9 mouse teratocarcinoma stem cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑1 in Undifferentiated D3 Mouse Cell Line by Flow Cytometry. Undifferentiated D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA‑1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2155C, filled histogram) or isotype control antibody (Catalog # IC015C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-1
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
  • References:
    1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
    2. Fox, N. et al. (1983) Cancer Res. 43:669.
  • Long Name:
    Stage-specific Embryonic Antigen-1
  • Alternate Names:
    alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1
Related Research Areas
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human/Mouse SSEA-1 PerCP-conjugated Antibody FAB2155C
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 Flow Mouse Anonymous 06/26/2017
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Flow Cytometry Human/Mouse SSEA-1 PerCP-conjugated Antibody FAB2155C
Flow Cytometry: Human/Mouse SSEA-1 PerCP-conjugated Antibody [FAB2155C]

Summary

ApplicationFlow Cytometry
Sample TestedCompensation Beads
SpeciesMouse

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