Human/Mouse SSEA-1 PE-conjugated Antibody

(8 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse SSEA-1.
  • Source
    Monoclonal Mouse IgM Clone # MC-480
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    F9 mouse teratocarcinoma stem cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-1
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
  • References:
    1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
    2. Fox, N. et al. (1983) Cancer Res. 43:669.
  • Long Name:
    Stage-specific Embryonic Antigen-1
  • Alternate Names:
    alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 8 of 8
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Sample Type
  1. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
    Authors: Sugawa F, Arauzo-Bravo M, Yoon J, Kim K, Aramaki S, Wu G, Stehling M, Psathaki O, Hubner K, Scholer H
    EMBO J, 2015;34(8):1009-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. 5-Aminoimidazole-4-carboxyamide ribonucleoside induces G(1)/S arrest and Nanog downregulation via p53 and enhances erythroid differentiation.
    Authors: Chae HD, Lee MR, Broxmeyer HE
    Stem Cells, 2012;30(2):140-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
    Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
    Biotechnol. Bioeng., 2009;102(6):1636-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro.
    Authors: Hayashi K, Surani MA
    Development, 2009;136(21):3549-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency.
    Authors: Hanna J, Markoulaki S, Schorderet P, Carey BW, Beard C, Wernig M, Creyghton MP, Steine EJ, Cassady JP, Foreman R, Lengner CJ, Dausman JA, Jaenisch R
    Cell, 2008;133(2):250-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
    Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
    Stem Cells, 2007;25(6):1393-401.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM PE-conjugated Control

Ctrl IC015P 1
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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