Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry, Cell Selection

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgM Clone # MC-480
View all SSEA-1 Primary Antibodies »

Product Specifications

Immunogen

F9 mouse teratocarcinoma stem cells

Specificity

Detects human and mouse SSEA-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human/Mouse SSEA‑1 PE‑conjugated Antibody

Detection of SSEA-1 antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of SSEA‑1 in Whole blood granulocytes cells by Flow Cytometry.

Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse SSEA‑1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: D3 mouse embryonic stem cell line and Whole blood granulocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References

  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Additional SSEA-1 Products

Product Documents for Human/Mouse SSEA‑1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SSEA‑1 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse SSEA‑1 PE‑conjugated Antibody

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