Human/Mouse SSEA-1 Antibody

Name: Human/Mouse SSEA-1 Antibody
Brand: R&D Systems
SKU: MAB2155-SP
Rating: 5 (1 reviews)

Catalog #: MAB2155
18 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2155
MAB2155-SP

Request a Quote Bulk Orders

2 Images

Product Details

Citations (18)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SSEA-1.

Source

Monoclonal Mouse IgM Clone # MC-480

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

F9 mouse teratocarcinoma stem cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Whole blood granulocytes

Immunoprecipitation

Ballou, B. et al. (1979) Science 206:844.

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry

View Larger

Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References

Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
Fox, N. et al. (1983) Cancer Res. 43:669.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse SSEA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
Filter your results:

Filter by:

The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level
Authors: S Farahi, S Hosseini, H Ghanbarian, SM Hashemi, M Salehi, S Hosseini
Stem Cells International, 2022;2022(0):9346767.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF
The H2B ubiquitin-protein ligase RNF40 is required for somatic cell reprogramming
Authors: W Xie, M Miehe, S Laufer, SA Johnsen
Cell Death Dis, 2020;11(4):287.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition
Authors: Y Wu, K Chen, G Xing, L Li, B Ma, Z Hu, L Duan, X Liu
Sci Adv, 2019;5(11):eaax7525.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
Stem Cell Res, 2017;20(0):105-114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
Oxid Med Cell Longev, 2017;2017(0):4745252.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation
Authors: M Klimczak, P Czerwi?ska, S Mazurek, B Soza?ska, P Biecek, A Mackiewicz, M Wiznerowic
Stem Cell Res, 2017;23(0):163-172.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
Cell Death Discov, 2017;3(0):16105.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
Oncotarget, 2017;8(17):28074-28092.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
Authors: Boo, Kyungjin, Bhin, Jinhyuk, Jeon, Yoon, Kim, Joomyung, Shin, Hi-Jai R, Park, Jong-Eun, Kim, Kyeongky, Kim, Chang Ro, Jang, Hyonchol, Kim, In-Hoo, Kim, V Narry, Hwang, Daehee, Lee, Ho, Baek, Sung Hee
Nat Commun, 2015;6(0):6810.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC
B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
Stem Cells, 2014;32(10):2626-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Pluripotent stem cells derived from mouse and human white mature adipocytes.
Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
Stem Cells Transl Med, 2014;3(2):161-71.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
BMC Vet Res, 2012;8(0):150.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry

Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
Nature, 2010;468(7325):824-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Aire regulates the expression of differentiation-associated genes and self-renewal of embryonic stem cells.
Authors: Gu B, Zhang J, Chen Q, Tao B, Wang W, Zhou Y, Chen L, Liu Y, Zhang M
Biochem. Biophys. Res. Commun., 2010;394(2):418-23.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
Stem Cells, 2008;26(4):874-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
Authors: Reed SA, Johnson SE
J. Cell. Physiol., 2008;215(2):329-36.
Species: Equine
Sample Types: Whole Cells
Applications: ICC

The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling.
Authors: Epping MT, Wang L, Edel MJ, Carlee L, Hernandez M, Bernards R
Cell, 2005;122(6):835-47.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC