Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Canine, Chicken, Equine, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MC-480
View all SSEA-1 Primary Antibodies »

Product Specifications

Immunogen

F9 mouse teratocarcinoma stem cells

Specificity

Detects human and mouse SSEA-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human/Mouse SSEA‑1 Antibody

SSEA‑1 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

SSEA‑1 in D3 Mouse Cell Line.

SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry.

Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence

Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Analysis of stem cell markers in tumor sample(A) Real-time quantitative PCR indicated there were no significant differences on stem cell markers expression between normal control animals and normoxia mice implanted with normoxia-treated cells; however, there were significant higher expression of stem cell markers in low oxygen feeding than control or normoxia feeding (*P > 0.05, *P < 0.05, One-way ANOVA). (B) Western blot analysis demonstrated that low oxygen feeding induced significantly increased levels of stem cell markers. (C) Gray value analysis of western-blot of B by Quantity One indicated the same results as real-time quantitative PCR (*P > 0.05, *P < 0.05, One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Time-dependent expression of GSC markers following hypoxia(A) Real-time quantitative PCR indicated time-dependent changes of stem cell markers before (con) and after hypoxia in U87 glioma cells. In general, 6 h after hypoxia, there was a significant increase of stem cell markers, which reached peak values at 9–12 h. (*P < 0.05, One-sample T Test). (B) Western blot analysis indicated a higher expression of stem cell markers after hypoxia for 12–48 h in U87 glioma cells. (C) Gray value analysis of Western blot in B by Quantity One indicated the expression of stem cell markers (SOX-2, OCT-4, KLF-4, Nanog, CD133, CD15, NESTIN and ABCG2) increased at least two-fold compared with control (*P < 0.05, One-sample T Test). (D) An increase expression of CD133, CD15 and NESTIN with a time-dependent manner after hypoxia (*P < 0.05, One-sample T Test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse SSEA‑1 Antibody

Application
Recommended Usage

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Whole blood granulocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Immunoprecipitation

Ballou, B. et al. (1979) Science 206:844.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2155 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References

  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Additional SSEA-1 Products

Product Documents for Human/Mouse SSEA‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse SSEA‑1 Antibody

For research use only

Citations for Human/Mouse SSEA‑1 Antibody

Customer Reviews for Human/Mouse SSEA‑1 Antibody (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human/Mouse SSEA-1 Antibody
    Name: Julieta O'Flaherty
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/11/2020
    I used to check whether SSEA-1 is expressed in my iPSCs and it was great.. no background signal
    Human/Mouse SSEA‑1 Antibody MAB2155
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23736260
    Species: Mouse
    Verified Customer | Posted 02/19/2015

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