Human/Mouse SSEA-1 Antibody MAB2155: R&D Systems

Human/Mouse SSEA-1 Antibody

(14 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse SSEA-1.
  • Source
    Monoclonal Mouse IgM Clone # MC-480
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    F9 mouse teratocarcinoma stem cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    D3 mouse embryonic stem cell line
  • Immunoprecipitation
    Ballou, B. et al. (1979) Science 206:844.
  • CyTOF-reported
    Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSEA-1
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
  • References:
    1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
    2. Fox, N. et al. (1983) Cancer Res. 43:669.
  • Long Name:
    Stage-specific Embryonic Antigen-1
  • Alternate Names:
    alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017;2017(0):4745252.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation
    Authors: M Klimczak, P Czerwi?ska, S Mazurek, B Soza?ska, P Biecek, A Mackiewicz, M Wiznerowic
    Stem Cell Res, 2017;23(0):163-172.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
    Authors: Boo, Kyungjin, Bhin, Jinhyuk, Jeon, Yoon, Kim, Joomyung, Shin, Hi-Jai R, Park, Jong-Eun, Kim, Kyeongky, Kim, Chang Ro, Jang, Hyonchol, Kim, In-Hoo, Kim, V Narry, Hwang, Daehee, Lee, Ho, Baek, Sung Hee
    Nat Commun, 2015;6(0):6810.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  6. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014;3(2):161-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014;32(10):2626-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  9. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010;468(7325):824-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Aire regulates the expression of differentiation-associated genes and self-renewal of embryonic stem cells.
    Authors: Gu B, Zhang J, Chen Q, Tao B, Wang W, Zhou Y, Chen L, Liu Y, Zhang M
    Biochem. Biophys. Res. Commun., 2010;394(2):418-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
    Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
    Stem Cells, 2008;26(4):874-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Type: Whole Cells
    Application: ICC
  13. The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling.
    Authors: Epping MT, Wang L, Edel MJ, Carlee L, Hernandez M, Bernards R
    Cell, 2005;122(6):835-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 14 Citations
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