Human/Mouse SSEA-1 Antibody

Catalog # Availability Size / Price Qty
MAB2155
MAB2155-SP
Human/Mouse SSEA-1 Antibody in Data
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Product Details
Citations (17)
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Reviews (1)

Human/Mouse SSEA-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunoprecipitation
Ballou, B. et al. (1979) Science 206:844.
 
CyTOF-reported
Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry SSEA‑1 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References
  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

Product Datasheets

Citations for Human/Mouse SSEA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. The H2B ubiquitin-protein ligase RNF40 is required for somatic cell reprogramming
    Authors: W Xie, M Miehe, S Laufer, SA Johnsen
    Cell Death Dis, 2020;11(4):287.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition
    Authors: Y Wu, K Chen, G Xing, L Li, B Ma, Z Hu, L Duan, X Liu
    Sci Adv, 2019;5(11):eaax7525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017;2017(0):4745252.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation
    Authors: M Klimczak, P Czerwi?ska, S Mazurek, B Soza?ska, P Biecek, A Mackiewicz, M Wiznerowic
    Stem Cell Res, 2017;23(0):163-172.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
    Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
    Cell Death Discov, 2017;3(0):16105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
    Authors: Boo, Kyungjin, Bhin, Jinhyuk, Jeon, Yoon, Kim, Joomyung, Shin, Hi-Jai R, Park, Jong-Eun, Kim, Kyeongky, Kim, Chang Ro, Jang, Hyonchol, Kim, In-Hoo, Kim, V Narry, Hwang, Daehee, Lee, Ho, Baek, Sung Hee
    Nat Commun, 2015;6(0):6810.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  9. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014;32(10):2626-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014;3(2):161-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Aire regulates the expression of differentiation-associated genes and self-renewal of embryonic stem cells.
    Authors: Gu B, Zhang J, Chen Q, Tao B, Wang W, Zhou Y, Chen L, Liu Y, Zhang M
    Biochem. Biophys. Res. Commun., 2010;394(2):418-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010;468(7325):824-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
    Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
    Stem Cells, 2008;26(4):874-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  16. The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling.
    Authors: Epping MT, Wang L, Edel MJ, Carlee L, Hernandez M, Bernards R
    Cell, 2005;122(6):835-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Human/Mouse SSEA-1 Antibody
By Anonymous on 12/11/2020
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used to check whether SSEA-1 is expressed in my iPSCs and it was great.. no background signal