Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Canine, Chicken, Equine, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MC-480
View all SSEA-1 Primary Antibodies »

Product Specifications

Immunogen

F9 mouse teratocarcinoma stem cells

Specificity

Detects human and mouse SSEA-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SSEA-1 Antibody (MC-480)

SSEA‑1 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

SSEA‑1 in D3 Mouse Cell Line.

SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry.

Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence

Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Analysis of stem cell markers in tumor sample(A) Real-time quantitative PCR indicated there were no significant differences on stem cell markers expression between normal control animals and normoxia mice implanted with normoxia-treated cells; however, there were significant higher expression of stem cell markers in low oxygen feeding than control or normoxia feeding (*P > 0.05, *P < 0.05, One-way ANOVA). (B) Western blot analysis demonstrated that low oxygen feeding induced significantly increased levels of stem cell markers. (C) Gray value analysis of western-blot of B by Quantity One indicated the same results as real-time quantitative PCR (*P > 0.05, *P < 0.05, One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse SSEA-1 by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Time-dependent expression of GSC markers following hypoxia(A) Real-time quantitative PCR indicated time-dependent changes of stem cell markers before (con) and after hypoxia in U87 glioma cells. In general, 6 h after hypoxia, there was a significant increase of stem cell markers, which reached peak values at 9–12 h. (*P < 0.05, One-sample T Test). (B) Western blot analysis indicated a higher expression of stem cell markers after hypoxia for 12–48 h in U87 glioma cells. (C) Gray value analysis of Western blot in B by Quantity One indicated the expression of stem cell markers (SOX-2, OCT-4, KLF-4, Nanog, CD133, CD15, NESTIN and ABCG2) increased at least two-fold compared with control (*P < 0.05, One-sample T Test). (D) An increase expression of CD133, CD15 and NESTIN with a time-dependent manner after hypoxia (*P < 0.05, One-sample T Test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Canine SSEA-1 by Flow Cytometry

Detection of Canine SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of fkisk0jukvze4fxockxs.jpg by Immunocytochemistry/ Immunofluorescence

Detection of fkisk0jukvze4fxockxs.jpg by Immunocytochemistry/ Immunofluorescence

Differentiation of human pluripotent stem cells (PSCs) into human primordial germ cell-like cells (hPGCLCs)(A) Timeline and culture strategy of in vitro hPGCLC differentiation.(B) The morphology of human embryonic stem cells (hESCs), embryoid bodies (EBs) and hPGCLC aggregates induced by ABR (activin A, BMP4 and retinoic acid) treatment.(C) The comparison of transcriptional profiles of hPGCLC aggregates by ABR treatment between H9 hESCs and human cord blood cell-derived induced pluripotent stem cells (CBiPSCs).(D) The expression of germline makers in hPGCLC aggregates derived from H9 hESCs and CBiPSCs.(E and F) (E) The expression levels of DDX4 and SSEA1 mRNA and (F) protein under ABR treatment.(G) Immunofluorescent staining of DDX4 and SSEA1 in hPGCLC aggregates.Mann-Whitney U test was used for statistical analysis.Bar graph represents mean ± SEM from at least three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004220312001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Immunohistochemistry

Detection of SSEA-1 by Immunohistochemistry

Isolation and identification of endometrial epithelial cells. (A) The schematic showed the procedure of isolation of endometrial epithelial cells and generation of an air–liquid interface (ALI) culture. (B) Identification of endometrial epithelial cells. Cells grown with Rho-associated protein kinase (ROCK)-modified medium on collagen type I rat tail–coated dishes exhibited a capacity to form colonies, which expressed cell surface antigen epithelial cellular adhesion molecule (EpCam) but not CD13 as determined by an immunocytochemistry assay with hematoxylin counterstaining. (C) Immunofluorescent staining for Ki67 or stage-specific embryonic antigen-1 (SSEA-1) (green) revealed that a subset of primary human endometrial epithelial cells expressed Ki67 or SSEA-1. (D) Immunoblotting assay confirmed the expression of indicated proteins of interest in native human endometrial biopsy tissues and isolated cell cultures of passage 0–3. (E) Immunoblotting assay confirmed stem cell marker expression of Nanog, Oct3/4, Sox 2, p63, c-Myc, and CD117 (c-kit) in native human endometrial biopsy tissues and isolated cell cultures of passages 0–4. (E′) Semi-quantitative analysis of the fold changes of the expression of proteins in (E) accessed by a densitometric assay. Compared with passage 0 (P0) cells, *P <0.05; **P <0.01 (analysis of variance). Scale bars = 100 μm (C) and 25 μm (C′) Image collected and cropped by CiteAb from the following open publication (https://stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0962-6), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Analysis of stem cell markers in tumor sample(A) Real-time quantitative PCR indicated there were no significant differences on stem cell markers expression between normal control animals and normoxia mice implanted with normoxia-treated cells; however, there were significant higher expression of stem cell markers in low oxygen feeding than control or normoxia feeding (*P > 0.05, *P < 0.05, One-way ANOVA). (B) Western blot analysis demonstrated that low oxygen feeding induced significantly increased levels of stem cell markers. (C) Gray value analysis of western-blot of B by Quantity One indicated the same results as real-time quantitative PCR (*P > 0.05, *P < 0.05, One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Western Blot

Detection of SSEA-1 by Western Blot

Time-dependent expression of GSC markers following hypoxia(A) Real-time quantitative PCR indicated time-dependent changes of stem cell markers before (con) and after hypoxia in U87 glioma cells. In general, 6 h after hypoxia, there was a significant increase of stem cell markers, which reached peak values at 9–12 h. (*P < 0.05, One-sample T Test). (B) Western blot analysis indicated a higher expression of stem cell markers after hypoxia for 12–48 h in U87 glioma cells. (C) Gray value analysis of Western blot in B by Quantity One indicated the expression of stem cell markers (SOX-2, OCT-4, KLF-4, Nanog, CD133, CD15, NESTIN and ABCG2) increased at least two-fold compared with control (*P < 0.05, One-sample T Test). (D) An increase expression of CD133, CD15 and NESTIN with a time-dependent manner after hypoxia (*P < 0.05, One-sample T Test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28427209), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Immunohistochemistry

Detection of SSEA-1 by Immunohistochemistry

Differentiation of human pluripotent stem cells (PSCs) into human primordial germ cell-like cells (hPGCLCs)(A) Timeline and culture strategy of in vitro hPGCLC differentiation.(B) The morphology of human embryonic stem cells (hESCs), embryoid bodies (EBs) and hPGCLC aggregates induced by ABR (activin A, BMP4 and retinoic acid) treatment.(C) The comparison of transcriptional profiles of hPGCLC aggregates by ABR treatment between H9 hESCs and human cord blood cell-derived induced pluripotent stem cells (CBiPSCs).(D) The expression of germline makers in hPGCLC aggregates derived from H9 hESCs and CBiPSCs.(E and F) (E) The expression levels of DDX4 and SSEA1 mRNA and (F) protein under ABR treatment.(G) Immunofluorescent staining of DDX4 and SSEA1 in hPGCLC aggregates.Mann-Whitney U test was used for statistical analysis.Bar graph represents mean ± SEM from at least three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004220312001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Immunocytochemistry/ Immunofluorescence

Detection of SSEA-1 by Immunocytochemistry/ Immunofluorescence

Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Flow Cytometry

Detection of SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SSEA-1 by Flow Cytometry

Detection of SSEA-1 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for SSEA-1 Antibody (MC-480)

Application
Recommended Usage

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Whole blood granulocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Immunoprecipitation

Ballou, B. et al. (1979) Science 206:844.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2155 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References

  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (MC-480)

Certificate of Analysis

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Product Specific Notices for SSEA-1 Antibody (MC-480)

For research use only

Citations for SSEA-1 Antibody (MC-480)

Customer Reviews for SSEA-1 Antibody (MC-480) (2)

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  • Human/Mouse SSEA-1 Antibody
    Name: Julieta O'Flaherty
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/11/2020
    I used to check whether SSEA-1 is expressed in my iPSCs and it was great.. no background signal
    SSEA-1 Antibody (MC-480) MAB2155
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23736260
    Species: Mouse
    Verified Customer | Posted 02/19/2015

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Protocols

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