Human/Mouse SWAP70 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SWAP70

SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators; in dendritic cells, it regulates the appearance of MHC II; and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96% aa identity with mouse SWAP70.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• Adaptor Proteins in the Akt Pathway
• B Cell Activation