Detection of Human and Mouse SWAP70 by Western Blot.
Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse SWAP70 Monoclonal Antibody (Catalog # MAB6735) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SWAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators; in dendritic cells, it regulates the appearance of MHC II; and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96% aa identity with mouse SWAP70.
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