Human/Mouse TLE3 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF5785X Datasheet / COA / SDS

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AF5785X-100UG

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Human/Mouse TLE3 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human TLE3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TLE3
His11-Asn200
Accession # Q04726

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TLE3

TLE3 (Transducin-Like Enhancer of Split 3; also ESG3 and GRG3) is an 84‑86 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed in the embryo, and in differentiating epithelial cells in the adult. TLE3 binds to a number of transcription factors as a heterooligomer, or to itself as a homotetramer. Oligomerization is essential for activity. Human TLE3is 772 amino acids (aa) in length. It contains a Gln(Q)-rich region that mediates oligomerization (aa 1‑131), a CCN domain that contains an NLS plus CKII and cdc2K kinase site (aa 199‑268), and seven WD repeats that mediate protein-protein interaction (aa 484‑771). Potentially there are multiple splice variants. Over aa 11‑200, human TLE3shares 99% aa identity wih mouse TLE3.

Long Name

Transducin-like Enhancer of Split 3

Entrez Gene IDs

7090 (Human); 21887 (Mouse); 84424 (Rat)

Alternate Names

ESG3; ESG3Enhancer of split groucho-like protein 3; ESGenhancer of split groucho 3; Grg3; HsT18976; KIAA1547FLJ39460; TLE3; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1); transducin-like enhancer protein 3