Human/Mouse VGF Antibody

Catalog # Availability Size / Price Qty
VGF in Human Brain.
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Human/Mouse VGF Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse VGF in direct ELISAs.
Monoclonal Mouse IgG3 Clone # 619707
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human VGF
Accession # O15240
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
1.7-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and perfusion fixed frozen sections of mouse brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

VGF in Human Brain. VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VGF

Neurosecretory protein VGF (VGF) is a 65 kDa secreted protein that is expressed in the brain, where it may be involved in the regulation of cell-cell interactions or in synaptogenesis during maturation of the nervous system. Human VGF is synthesized as a 615 amino acid (aa) precursor that contains a 22 aa signal sequence and a 593 aa mature chain. The region including aa 353-447 is Asp/Glu-rich and thus an acidic region. Human VGF shares 87% aa sequence identity to mouse VGF.

Long Name
Neurosecretory Protein VGF
Entrez Gene IDs
7425 (Human)
Alternate Names
neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible; VGF

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse VGF Antibody
By Anonymous on 07/07/2022
Application: IHC Sample Tested: Brain tissue Species: Mouse