Human/Mouse VGF Antibody

R&D Systems | Catalog # MAB6317

R&D Systems
Discontinued Product
MAB6317 has been discontinued. View all VGF products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 619707
View all VGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VGF
Thr554-Pro615
Accession # O15240

Specificity

Detects human and mouse VGF in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human/Mouse VGF Antibody

VGF in Human Brain.

VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse VGF Antibody

Application
Recommended Usage

Immunohistochemistry

1.7-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and perfusion fixed frozen sections of mouse brain (cortex)

Reviewed Applications

Read 1 review rated 5 using MAB6317 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VGF

Neurosecretory protein VGF (VGF) is a 65 kDa secreted protein that is expressed in the brain, where it may be involved in the regulation of cell-cell interactions or in synaptogenesis during maturation of the nervous system. Human VGF is synthesized as a 615 amino acid (aa) precursor that contains a 22 aa signal sequence and a 593 aa mature chain. The region including aa 353-447 is Asp/Glu-rich and thus an acidic region. Human VGF shares 87% aa sequence identity to mouse VGF.

Long Name

Neurosecretory Protein VGF

Alternate Names

neuro-endocrine specific protein VGF, neurosecretory protein VGF, VGF nerve growth factor inducible

Entrez Gene IDs

7425 (Human)

Gene Symbol

VGF

UniProt

O15240

Additional VGF Products

Product Documents for Human/Mouse VGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse VGF Antibody

For research use only

Related Research Areas

Nutrition and Appetite

Customer Reviews for Human/Mouse VGF Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse VGF Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse VGF Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 07/07/2022
    Human/Mouse VGF Antibody MAB6317

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies