Human MRGX2 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry.
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Product Details
Citations (1)

Human MRGX2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants.
Monoclonal Mouse IgG2A Clone # 477533
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human MRGX2
Accession # Q96LB1
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MRGX2 antibody in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP antibody by Flow Cytometry. View Larger

Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with A) human MRGX2 or B) irrelevant transfectant and eGFP, was stained with Alexa Fluor® 647-conjugated Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # IC4727R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MRGX2

MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins, but has no ortholog in rodents. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.

Long Name
Mas-related G Protein-coupled Receptor Member X2
Entrez Gene IDs
117194 (Human); 243978 (Mouse)
Alternate Names
MAS-related GPR, member X2; mas-related G-protein coupled receptor member X2; MRGPRX2; MRGX2; MRGX2G protein-coupled receptor MRGX2

Product Datasheets

Citation for Human MRGX2 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated monoosteophils
    Authors: Z Zhang, K Le, D La Placa, B Armstrong, MM Miller, JE Shively
    Bone Rep, 2020;12(0):100237.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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