|Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human MRGX2 and eGFP was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.|
Intracellular Staining by Flow Cytometry
|Detection of MRGX2 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins, but has no ortholog in rodents. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.
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