Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 477533
View all MRGX2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human MRGX2
Met1-Val330
Accession # Q96LB1

Specificity

Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human MRGX2 Antibody

Detection of MRGX2 antibody in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP antibody by Flow Cytometry.

Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human MRGX2 and eGFP was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Detection of MRGX2 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of MRGX2 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human MRGX2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human MRGX2

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MRGX2

MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.

Long Name

Mas-related G Protein-coupled Receptor Member X2

Alternate Names

MRGPRX2

Entrez Gene IDs

117194 (Human); 243978 (Mouse)

Gene Symbol

MRGPRX2

UniProt

Q96LB1

Additional MRGX2 Products

Product Documents for Human MRGX2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MRGX2 Antibody

For research use only

Citations for Human MRGX2 Antibody

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