Detection of Myosin Heavy Chain in Three-day Differentiated C2C12 Mouse Cell Line by Flow Cytometry. Three-day differentiated C2C12 mouse myoblast cell line was stained with Mouse Anti-Human Myosin Heavy Chain APC‑conjugated Monoclonal Antibody (Catalog # IC4470A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Myosin Heavy Chain
Skeletal muscle Myosin or Myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of Actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy Meromyosin, which includes the S1 motor domain (head region) and first third of the coiled-coil domain, and light Meromyosin, which includes the C-terminal two thirds of the coiled-coil domain.
DFNA4; FLJ13881; FLJ43092; KIAA2034DKFZp667A1311; MHC16; MYH14 variant protein; MYH17; MYHC; Myosin heavy chain 14; Myosin heavy chain, non-muscle IIc; myosin; myosin, heavy chain 14; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C
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