Myosin Heavy Chain Antibody

(16 citations)
(1 Review)
  
  • Specificity
    Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
  • Source
    Monoclonal Mouse IgG2B Clone # MF20
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chicken pectoralis-derived Myosin
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Stains, C.I., et al. (2012) Chem Biol. 19:210.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myosin Heavy Chain
Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.
  • Alternate Names:
    DFNA4; FLJ13881; FLJ43092; KIAA2034DKFZp667A1311; MHC16; MYH14 variant protein; MYH17; MYHC; Myosin heavy chain 14; Myosin Heavy Chain; Myosin heavy chain, non-muscle IIc; myosin; myosin, heavy chain 14; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways
    Authors: F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand
    Cell Rep, 2017;18(10):2320-2330.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability
    Authors: J Ueki, M Nakamori, M Nakamura, M Nishikawa, Y Yoshida, A Tanaka, A Morizane, M Kamon, T Araki, MP Takahashi, A Watanabe, N Inagaki, H Sakurai
    Sci Rep, 2017;7(0):42522.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation
    Authors: DS Han, WS Yang, TW Kao
    Int J Med Sci, 2017;14(5):434-443.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor
    Authors: S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
    Authors: G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li
    Nat Commun, 2017;8(1):589.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation
    Sci Rep, 2016;6(0):28829.
    Species: Human
    Sample Type: Protein
    Application: WB
  7. Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1
    Sci Rep, 2016;6(0):32084.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma
    Authors: A Kim, M Im, MJ Gu, JY Ma
    Sci Rep, 2016;6(0):24214.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
  10. Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
    Authors: Vleeshouwer-Neumann T, Phelps M, Bammler T, Macdonald J, Jenkins I, Chen E
    PLoS ONE, 2015;10(12):e0144320.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  11. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014;184(4):1152-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Molecular ties between the cell cycle and differentiation in embryonic stem cells.
    Authors: Li, Victor C, Kirschner, Marc W
    Proc Natl Acad Sci U S A, 2014;111(26):9503-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  13. Myoblasts Derived From Normal hESCs and Dystrophic hiPSCs Efficiently Fuse With Existing Muscle Fibers Following Transplantation.
    Authors: Goudenege S, Lebel C, Huot N, Dufour C, Fujii I, Gekas J, Rousseau J, Tremblay J
    Mol Ther, 2012;20(11):2153-67.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
    Authors: Ono Y, Masuda S, Nam HS
    J. Cell. Sci., 2012;125(0):1309-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  15. Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
    Authors: Joe MK, Kee C, Tomarev SI
    J. Biol. Chem., 2012;287(16):13216-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  16. Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
    Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
    Diabetes, 0;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: Simple Western
Expand to show all 16 Citations
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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  Very Good
 ICC/IF Human Anonymous 05/10/2017
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedSkeletal muscle tissue
SpeciesHuman

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