Myosin Heavy Chain Antibody

Catalog # Availability Size / Price Qty
MAB4470
MAB4470-SP

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Myosin Heavy Chain in C2C12 Mouse Cell Line.
3 Images
Product Details
Citations (78)
FAQs
Supplemental Products
Reviews (7)

Myosin Heavy Chain Antibody Summary

Specificity
Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
Source
Monoclonal Mouse IgG2B Clone # MF20
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chicken pectoralis-derived Myosin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Stains, C.I., et al. (2012) Chem Biol. 19:210.
 
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Myosin Heavy Chain antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Myosin Heavy Chain antibody in Human Skeletal Muscle by Immunohistochemistry (IHC-P). View Larger

Myosin Heavy Chain in Human Skeletal Muscle. Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Myosin Heavy Chain antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Myosin Heavy Chain in Mouse Skeletal Muscle. Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myosin Heavy Chain

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Alternate Names
DFNA4; FLJ13881; FLJ43092; KIAA2034DKFZp667A1311; MHC16; MYH14 variant protein; MYH17; MYHC; Myosin heavy chain 14; Myosin Heavy Chain; Myosin heavy chain, non-muscle IIc; myosin; myosin, heavy chain 14; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C

Product Datasheets

You must select a language.

x

Citations for Myosin Heavy Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

78 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells
    Authors: I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur
    Science Advances, 2022;8(14):eabj4928.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  2. Knockdown of CDR1as Decreases Differentiation of Goat Skeletal Muscle Satellite Cells via Upregulating miR-27a-3p to Inhibit ANGPT1
    Authors: B Kyei, E Odame, L Li, L Yang, S Zhan, J Li, Y Chen, D Dai, J Cao, J Guo, T Zhong, L Wang, H Zhang
    Genes, 2022;13(4):.
    Species: Goat
    Sample Types: Transfected Whole Cells
    Applications: ICC
  3. COPS3 AS lncRNA enhances myogenic differentiation and maintains fast-type myotube phenotype
    Authors: ZZ He, T Zhao, N Qimuge, T Tian, W Yan, X Yi, J Jin, R Cai, T Yu, G Yang, W Pang
    Cellular Signalling, 2022;95(0):110341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Tceal5 and Tceal7 Function in C2C12 Myogenic Differentiation via Exosomes in Fetal Bovine Serum
    Authors: A Sawada, T Yamamoto, T Sato
    International Journal of Molecular Sciences, 2022;23(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Simple derivation of skeletal muscle from human pluripotent stem cells using temperature-sensitive Sendai virus vector
    Authors: GW Tan, T Kondo, K Imamura, M Suga, T Enami, A Nagahashi, K Tsukita, I Inoue, J Kawaguchi, T Shu, H Inoue
    Journal of Cellular and Molecular Medicine, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. The Effects of Muscle Cell Aging on Myogenesis
    Authors: A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier
    International Journal of Molecular Sciences, 2021;22(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle
    Authors: K Yoshioka, H Nagahisa, F Miura, H Araki, Y Kamei, Y Kitajima, D Seko, J Nogami, Y Tsuchiya, N Okazaki, A Yonekura, S Ohba, Y Sumita, K Chiba, K Ito, I Asahina, Y Ogawa, T Ito, Y Ohkawa, Y Ono
    Science Advances, 2021;7(24):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. An integrative transcriptome study reveals Ddit4/Redd1 as a key regulator of cancer cachexia in rodent models
    Authors: M Niu, L Li, Z Su, L Wei, W Pu, C Zhao, Y Ding, J Wazir, W Cao, S Song, Q Gao, H Wang
    Cell Death & Disease, 2021;12(7):652.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  9. M2 Macrophage-derived exosomal miR-501 contributes to pubococcygeal muscle regeneration
    Authors: M Zhou, B Li, C Liu, M Hu, J Tang, J Min, J Cheng, L Hong
    International immunopharmacology, 2021;101(0):108223.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
    Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
    Toxicology reports, 2021;8(0):1513-1520.
    Species: Mouse
    Sample Types: Embryoid Bodies
    Applications: ICC
  11. The ubiquitin ligase Ozz decreases the replacement rate of embryonic myosin in myofibrils
    Authors: E Ichimura, K Ojima, S Muroya, T Suzuki, K Kobayashi, T Nishimura
    Physiological Reports, 2021;9(17):e15003.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  12. Wharton's Jelly-Derived Mesenchymal Stem Cells Reduce Fibrosis in a Mouse Model of Duchenne Muscular Dystrophy by Upregulating microRNA 499
    Authors: SE Park, JB Jeong, SJ Oh, SJ Kim, H Kim, A Choi, SJ Choi, SY Oh, GH Ryu, J Lee, HB Jeon, JW Chang
    Biomedicines, 2021;9(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control
    Authors: B Lee, S Lee, Y Lee, Y Park, J Shim
    International Journal of Molecular Sciences, 2021;22(13):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  14. VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway
    Authors: M Ling, L Quan, X Lai, L Lang, F Li, X Yang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang
    International Journal of Molecular Sciences, 2021;22(24):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. beta-Hydroxy-beta-Methylbutyrate Supplementation Promotes Antitumor Immunity in an Obesity Responsive Mouse Model of Pancreatic Ductal Adenocarcinoma
    Authors: MF Coleman, KA Liu, AJ Pfeil, SK Etigunta, X Tang, S Fabela, LM Lashinger, Z Cui, SD Hursting
    Cancers, 2021;13(24):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Neural stemness contributes to cell tumorigenicity
    Authors: L Xu, M Zhang, L Shi, X Yang, L Chen, N Cao, A Lei, Y Cao
    Cell & bioscience, 2021;11(1):21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test
    Authors: RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma
    Toxicology and Applied Pharmacology, 2021;433(0):115781.
    Species: Mouse
    Sample Types: Embryonic Bodies
    Applications: ICC
  18. Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration
    Authors: X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang
    Disease Models & Mechanisms, 2021;14(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia
    Authors: K Xie, H Xiong, W Xiao, Z Xiong, W Hu, J Ye, N Xu, J Shi, C Yuan, Z Chen, D Miao, X Zhang, H Yang
    Cancer Cell International, 2021;21(1):627.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway
    Authors: Y She, C Li, T Jiang, S Lei, S Zhou, H Shi, R Chen
    Frontiers in Physiology, 2021;12(0):659272.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors
    Authors: Y Nakatake, SBH Ko, AA Sharov, S Wakabayash, M Murakami, M Sakota, N Chikazawa, C Ookura, S Sato, N Ito, M Ishikawa-H, SS Mak, LM Jakt, T Ueno, K Hiratsuka, M Matsushita, SK Goparaju, T Akiyama, KI Ishiguro, M Oda, N Gouda, A Umezawa, H Akutsu, K Nishimura, R Matoba, O Ohara, MSH Ko
    Cell Rep, 2020;31(7):107655.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Urolithin A exerts antiobesity effects through enhancing adipose tissue thermogenesis in mice
    Authors: B Xia, XC Shi, BC Xie, MQ Zhu, Y Chen, XY Chu, GH Cai, M Liu, SZ Yang, GA Mitchell, WJ Pang, JW Wu
    PLoS Biol., 2020;18(3):e3000688.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. LMNA Mutations G232E and R482L Cause Dysregulation of Skeletal Muscle Differentiation, Bioenergetics, and Metabolic Gene Expression Profile
    Authors: EV Ignatieva, OA Ivanova, MY Komarova, NV Khromova, DE Polev, AA Kostareva, A Sergushich, RI Dmitrieva
    Genes (Basel), 2020;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Daily Oral Administration of Protease-Treated Royal Jelly Protects Against Denervation-Induced Skeletal Muscle Atrophy
    Authors: T Shirakawa, A Miyawaki, T Matsubara, N Okumura, H Okamoto, N Nakai, T Rojasawast, K Morikawa, A Inoue, A Goto, A Washio, T Tsujisawa, T Kawamoto, S Kokabu
    Nutrients, 2020;12(10):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ICC
  25. The calmodulin redox sensor controls myogenesis
    Authors: AW Steil, JW Kailing, CJ Armstrong, DG Walgenbach, JC Klein
    PLoS ONE, 2020;15(9):e0239047.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling
    Authors: A Knyazeva, A Khudiakov, R Vaz, A Muravyev, K Sukhareva, T Sejersen, A Kostareva
    Genes (Basel), 2020;11(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix
    Authors: J Lee, A Sutani, R Kaneko, J Takeuchi, T Sasano, T Kohda, K Ihara, K Takahashi, M Yamazoe, T Morio, T Furukawa, F Ishino
    Nat Commun, 2020;11(1):4283.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Corylifol A from Psoralea corylifolia L. Enhances Myogenesis and Alleviates Muscle Atrophy
    Authors: Y Han, H Lee, H Li, JH Ryu
    Int J Mol Sci, 2020;21(5):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration
    Authors: C Imashiro, M Hirano, T Morikura, Y Fukuma, K Ohnuma, Y Kurashina, S Miyata, K Takemura
    Sci Rep, 2020;10(1):9468.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Expression patterns of regulatory lncRNAs and miRNAs in muscular atrophy models induced by starvation in vitro and in vivo
    Authors: S Lei, Y She, J Zeng, R Chen, S Zhou, H Shi
    Mol Med Rep, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens
    Authors: Y Nihashi, K Umezawa, S Shinji, Y Hamaguchi, H Kobayashi, T Kono, T Ono, H Kagami, T Takaya
    Sci Rep, 2019;9(1):16527.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: ICC
  32. CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Authors: H Morisaka, K Yoshimi, Y Okuzaki, P Gee, Y Kunihiro, E Sonpho, H Xu, N Sasakawa, Y Naito, S Nakada, T Yamamoto, S Sano, A Hotta, J Takeda, T Mashimo
    Nat Commun, 2019;10(1):5302.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. The SMYD3 methyltransferase promotes myogenesis by activating the myogenin regulatory network
    Authors: R Codato, M Perichon, A Divol, E Fung, A Sotiropoul, A Bigot, JB Weitzman, S Medjkane
    Sci Rep, 2019;9(1):17298.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis
    Authors: G Picchiarel, M Demestre, A Zuko, M Been, J Higelin, S Dieterlé, MA Goy, M Mallik, C Sellier, J Scekic-Zah, L Zhang, A Rosenbohm, C Sijlmans, A Aly, S Mersmann, I Sanjuan-Ru, A Hübers, N Messaddeq, M Wagner, N van Bakel, AL Boutillier, A Ludolph, C Lagier-Tou, TM Boeckers, L Dupuis, E Storkebaum
    Nat. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells
    Authors: SY Lee, J Yang, JH Park, HK Shin, WJ Kim, SY Kim, EJ Lee, I Hwang, CS Lee, J Lee, HS Kim
    Mol. Ther., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  36. Skeletal Muscle Atrophy Was Alleviated by Salidroside Through Suppressing Oxidative Stress and Inflammation During Denervation
    Authors: Z Huang, Q Fang, W Ma, Q Zhang, J Qiu, X Gu, H Yang, H Sun
    Front Pharmacol, 2019;10(0):997.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  37. Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise
    Authors: Y Zhang, Y Liu, X Bi, C Hu, F Ding, W Ding
    Mediators Inflamm., 2019;2019(0):2789014.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs
    Authors: Y Komatsu, D Takeuchi, T Tokunaga, H Sakurai, A Makino, T Honda, Y Ikeda, K Tomonaga
    Mol Ther Methods Clin Dev, 2019;14(0):47-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Increase in HDAC9 suppresses myoblast differentiation via epigenetic regulation of autophagy in hypoxia
    Authors: Z Zhang, L Zhang, Y Zhou, L Li, J Zhao, W Qin, Z Jin, W Liu
    Cell Death Dis, 2019;10(8):552.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  40. MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In�Vivo
    Authors: LR Fiedler, K Chapman, M Xie, E Maifoshie, M Jenkins, PA Golforoush, M Bellahcene, M Noseda, D Faust, A Jarvis, G Newton, MA Paiva, M Harada, DJ Stuckey, W Song, J Habib, P Narasimham, R Aqil, D Sanmugalin, R Yan, L Pavanello, M Sano, SC Wang, RD Sampson, S Kanayagana, GE Taffet, LH Michael, ML Entman, TH Tan, SE Harding, CMR Low, C Tralau-Ste, T Perrior, MD Schneider
    Cell Stem Cell, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
    Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
    Stem Cells Int, 2019;2019(0):5690345.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  42. p300 mediates muscle wasting in Lewis lung carcinoma
    Authors: YP Li, TK Sin, JZ Zhu, G Zhang
    Cancer Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  43. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy
    Authors: MC Estañ, E Fernández-, MS Zaki, MI Esteban, S Donkervoor, C Hawkins, JA Caparros-M, D Saade, Y Hu, V Bolduc, KR Chao, J Nevado, A Lamuedra, R Largo, G Herrero-Be, J Regadera, C Hernandez-, EF Tizzano, V Martinez-G, JJ Carvajal, R Zong, DL Nelson, GA Otaify, S Temtamy, M Aglan, M Issa, CG Bönnemann, P Lapunzina, G Yoon, VL Ruiz-Perez
    Nat Commun, 2019;10(1):797.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  44. Epigenetic regulation of the PTEN-AKT-RAC1 axis by G9a is critical for tumor growth in alveolar rhabdomyosarcoma
    Authors: AV Bhat, M Palanicham, VK Rao, L Pignata, HJ Lim, S Suriyamurt, KTE Chang, VK Lee, E Guccione, R Taneja
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  45. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects
    Authors: J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi
    Cell Rep, 2019;26(9):2494-2508.e7.
  46. IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway
    Authors: D Liu, X Qiao, Z Ge, Y Shang, Y Li, W Wang, M Chen, S Si, SZ Chen
    Skelet Muscle, 2019;9(1):8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  47. Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs
    Authors: D Abbadi, M Yang, DM Chenette, JJ Andrews, RJ Schneider
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  48. Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia
    Authors: R Chen, J Xu, Y She, T Jiang, S Zhou, H Shi, C Li
    Int. J. Mol. Med., 2018;41(5):2565-2572.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  49. Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells
    Authors: R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin
    Stem Cells Dev., 2018;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P
  50. Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration
    Authors: M Koide, Y Hagiwara, M Tsuchiya, M Kanzaki, H Hatakeyama, Y Tanaka, T Minowa, T Takemura, A Ando, T Sekiguchi, Y Yabe, E Itoi
    Tohoku J. Exp. Med., 2018;244(1):15-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13
    Authors: B Kalbe, M Osterloh, VM Schulz, J Altmüller, C Becker, S Osterloh, H Hatt
    Arch. Biochem. Biophys., 2018;645(0):72-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. HSP90 modulates the myosin replacement rate in myofibrils
    Authors: K Ojima, E Ichimura, T Suzuki, M Oe, S Muroya, T Nishimura
    Am. J. Physiol., Cell Physiol., 2018;0(0):.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Pannexin 1 inhibits rhabdomyosarcoma progression through a mechanism independent of its canonical channel function
    Authors: X Xiang, S Langlois, ME St-Pierre, JF Barré, D Grynspan, B Purgina, KN Cowan
    Oncogenesis, 2018;7(11):89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons
    Authors: T Osaki, SGM Uzel, RD Kamm
    Sci Adv, 2018;4(10):eaat5847.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  55. The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts
    Authors: H Sakane, K Akasaki
    Biol. Pharm. Bull., 2018;41(8):1186-1193.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients
    Authors: M Wattin, L Gaweda, P Muller, M Baritaud, C Scholtes, C Lozano, K Gieseler, C Kretz-Remy
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  57. The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice
    Authors: T Sasaki, A Kuboyama, M Mita, S Murata, M Shimizu, J Inoue, K Mori, R Sato
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  58. Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors
    Authors: F Sakai-Take, A Narita, S Masuda, T Wakamatsu, N Watanabe, T Nishiyama, K Nogami, M Blanc, S Takeda, Y Miyagoe-Su
    Sci Rep, 2018;8(1):6555.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  59. R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways
    Authors: F Lacour, E Vezin, CF Bentzinger, MC Sincennes, L Giordani, A Ferry, R Mitchell, K Patel, MA Rudnicki, MC Chaboissie, AA Chassot, F Le Grand
    Cell Rep, 2017;18(10):2320-2330.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  60. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor
    Authors: S Kokabu, C Nakatomi, T Matsubara, Y Ono, WN Addison, JW Lowery, M Urata, AM Hudnall, S Hitomi, M Nakatomi, T Sato, K Osawa, T Yoda, V Rosen, E Jimi
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  61. Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
    Authors: G Zhang, Z Liu, H Ding, Y Zhou, HA Doan, KWT Sin, ZJ Zhu, R Flores, Y Wen, X Gong, Q Liu, YP Li
    Nat Commun, 2017;8(1):589.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  62. Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation
    Authors: DS Han, WS Yang, TW Kao
    Int J Med Sci, 2017;14(5):434-443.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  63. NF?B signaling in alveolar rhabdomyosarcoma
    Authors: MM Cleary, A Mansoor, T Settelmeye, Y Ijiri, KJ Ladner, MN Svalina, BP Rubin, DC Guttridge, C Keller
    Dis Model Mech, 2017;10(9):1109-1115.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  64. Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability
    Authors: J Ueki, M Nakamori, M Nakamura, M Nishikawa, Y Yoshida, A Tanaka, A Morizane, M Kamon, T Araki, MP Takahashi, A Watanabe, N Inagaki, H Sakurai
    Sci Rep, 2017;7(0):42522.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  65. Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy
    Authors: H Lee, SJ Lee, GU Bae, NI Baek, JH Ryu
    Int J Mol Sci, 2017;18(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  66. Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation
    Sci Rep, 2016;6(0):28829.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  67. Activin A induces skeletal muscle catabolism via p38? mitogen-activated protein kinase
    Authors: Yi-Ping Li
    J Cachexia Sarcopenia Muscle, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  68. Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma
    Authors: A Kim, M Im, MJ Gu, JY Ma
    Sci Rep, 2016;6(0):24214.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation
    Authors: Hiroyuki Tsutsui
    Am. J. Physiol., Cell Physiol., 2016;0(0):ajpcell.00090.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  70. CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
  71. Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1
    Sci Rep, 2016;6(0):32084.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  72. Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
    Authors: Vleeshouwer-Neumann T, Phelps M, Bammler T, Macdonald J, Jenkins I, Chen E
    PLoS ONE, 2015;10(12):e0144320.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  73. Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
    Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
    Am J Pathol, 2014;184(4):1152-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  74. Molecular ties between the cell cycle and differentiation in embryonic stem cells.
    Authors: Li, Victor C, Kirschner, Marc W
    Proc Natl Acad Sci U S A, 2014;111(26):9503-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  75. Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
    Authors: Ono Y, Masuda S, Nam HS
    J. Cell. Sci., 2012;125(0):1309-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  76. Myoblasts Derived From Normal hESCs and Dystrophic hiPSCs Efficiently Fuse With Existing Muscle Fibers Following Transplantation.
    Authors: Goudenege S, Lebel C, Huot N, Dufour C, Fujii I, Gekas J, Rousseau J, Tremblay J
    Mol Ther, 2012;20(11):2153-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  77. Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
    Authors: Joe MK, Kee C, Tomarev SI
    J. Biol. Chem., 2012;287(16):13216-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  78. Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
    Authors: Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J
    Diabetes, 0;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Simple Western

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Myosin Heavy Chain Antibody

Average Rating: 4.7 (Based on 7 Reviews)

5 Star
71.43%
4 Star
28.57%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Myosin Heavy Chain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Myosin Heavy Chain Antibody
By Anonymous on 03/29/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Muscle tissue Species: Human

Myosin Heavy Chain Antibody
By Anonymous on 08/02/2021
Application: Immunohistochemistry Sample Tested: Muscle tissue Species: Mouse

Immunofluorescence, mouse muscle tissue. On the photo: Myosin Heavy Chain Antibody in red, GAP43 in green.


Myosin Heavy Chain Antibody
By Anonymous on 12/04/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Myotubes Species: horse

Myosin Heavy Chain Antibody
By Anonymous on 10/29/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skeletal muscle Species: Mouse

Myosin Heavy Chain Antibody
By Anonymous on 10/23/2020
Application: Immunohistochemistry Sample Tested: Heart tissue Species: Mouse

Heart tissue was fixed with paraformaldehyde and embedded with paraffin. The section was pre-treated with heart induced antigen retrieval and blocked with 10% goat serum and 3% BSA. Then the section was incubated with MAB4470 with a concentration of 10 ug/mL at 4C overnight and incubated with anti-mouse Alexa Fluo 488 secondary antiobody for 1 hour at room temperature. Nuclei were stained with DAPI.


Myosin Heavy Chain Antibody
By Anonymous on 06/06/2019
Application: Western Blot Sample Tested: C2C12 mouse myoblast cell line,Differentiated C2C12 myoblast cells Species: Mouse

Myosin Heavy Chain Antibody
By Michelle Chen on 05/10/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skeletal muscle tissue Species: Human