Human Ninjurin-1 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Ninjurin-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ninjurin-2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 758926
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Ninjurin-1
    Accession # Q92982
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Ninjurin‑1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Ninjurin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB51051G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ninjurin-1

Ninjurin-1 (Nerve Injury-induced Protein 1) is a 20-22 kDa phosphoprotein member of the Ninjurin family of transmembrane (TM) proteins. It is either constitutively or inducibly expressed by Schwann cells, PNS neuron satellite cells, neutrophils, endothelial cells, macrophages, podocytes and hepatocytes, and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. With respect to human Ninjurin-2, which has only 65 aa in it N-terminal ECD, human Ninjurin-1 shares 51% aa sequence identity with human Ninjurin-2.

  • Entrez Gene IDs:
    4814 (Human); 18081 (Mouse); 25338 (Rat)
  • Alternate Names:
    Nerve injury-induced protein 1; nerve injury-induced protein-1; NIN1; NINJ1; ninjurin 1; NINJURIN; Ninjurin1; ninjurin-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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