Detection of Ninjurin‑1 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Ninjurin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB51051G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Ninjurin-1 (Nerve Injury-induced Protein 1) is a 20-22 kDa phosphoprotein member of the Ninjurin family of transmembrane (TM) proteins. It is either constitutively or inducibly expressed by Schwann cells, PNS neuron satellite cells, neutrophils, endothelial cells, macrophages, podocytes and hepatocytes, and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. With respect to human Ninjurin-2, which has only 65 aa in it N-terminal ECD, human Ninjurin-1 shares 51% aa sequence identity with human Ninjurin-2.
Have you used Human Ninjurin-1 Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.