Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Bioassay

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 134591
View all NKG2C/CD159c Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94

Specificity

Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NKG2C/CD159c PE‑conjugated Antibody

Detection of NKG2C/CD159c antibody in Human PBMCs antibody by Flow Cytometry.

Detection of NKG2C/CD159c in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c PE-conjugated Monoclonal Antibody (Catalog # FAB138P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Enhancement of cytokine‐driven IFN‐ gamma production within CD56bright, CD56dimNKG2C−CD57− and CD56dimNKG2C+CD57− NK cells after vaccination. (A) Frequencies of IFN‐ gamma producing NK cells after HCC stimulation of PBMC at baseline (0) and up to 24 weeks post TIV vaccination. (B) Gating strategy showing CD57 and NKG2C‐defined subsets. (C, D) Subset distribution for NK cell IFN‐ gamma production after HCC stimulation of pre (0 weeks) and post‐vaccination (4, 12, 24 weeks) samples from subjects receiving (C) TIV and (D) DTPiP. Dots represent the frequency of IFN‐ gamma + NK cells for each subject and horizontal lines represent median frequency for each group. Statistical analysis was performed on paired samples using Wilcoxon signed‐rank test, *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28383105), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human NKG2C/CD159c PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 4 reviews rated 4.3 using FAB138P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Long Name

Natural Killer G2C

Alternate Names

CD159c, KLRC2, NKG2-C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Gene Symbol

KLRC2

Additional NKG2C/CD159c Products

Product Documents for Human NKG2C/CD159c PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NKG2C/CD159c PE‑conjugated Antibody

For research use only

Citations for Human NKG2C/CD159c PE‑conjugated Antibody

Customer Reviews for Human NKG2C/CD159c PE‑conjugated Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
75%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 07/26/2022
  • Human NKG2C/CD159c Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMCs and Human PBMC
    Species: Human
    Verified Customer | Posted 05/18/2020
    SSC-A against NKG2C in lymphocytes and monocytes.
    Human NKG2C/CD159c PE‑conjugated Antibody FAB138P
  • Human NKG2C/CD159c PE-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Natural killer cells
    Species: Human
    Verified Customer | Posted 07/05/2018
  • Human NKG2C/CD159c Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 12/12/2016
    FACS plots gated on total NK cells and plotted for NKG2A v NKG2C. Clear separation of NKG2A and NKG2C subsets. Second plot shows histogram of NKG2C staining. NKG2C antibody used at 1:15 concentration.
    Cryopreserved human PBMC thawed and ran immediately after thawing. Surface stained for CD56, CD3, NKG2A and NKG2C. Fixed with BD Fix/perm before running on a LSRFortessa.
    Human NKG2C/CD159c PE‑conjugated Antibody FAB138P

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