Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody

  (7 citations)     
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Citations (7)
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  • Species Reactivity
    Human
  • Specificity
    Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
  • Source
    Monoclonal Mouse IgG1 Clone # 134591
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB138G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2C/CD159c
NKG2C, also known as hemoglobin scavenger receptor, is a type II transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region.
  • Long Name:
    Natural Killer G2C
  • Entrez Gene IDs:
    3822 (Human); 16642 (Mouse)
  • Alternate Names:
    CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Systems Vaccinology Identifies an Early Innate Immune Signature as a Correlate of Antibody Responses to the Ebola Vaccine rVSV-ZEBOV
    Authors: A Rechtien, L Richert, H Lorenzo, G Martrus, B Hejblum, C Dahlke, R Kasonta, M Zinser, H Stubbe, U Matschl, A Lohse, V Krähling, M Eickmann, S Becker,, R Thiébaut, M Altfeld, M Addo
    Cell Rep, 2017;20(9):2251-2261.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. KIR2DL3 and KIR2DL1 show similar impact on licensing of human NK cells.
    Authors: Sim M, Stowell J, Sergeant R, Altmann D, Long E, Boyton R
    Eur J Immunol, 2016;46(1):185-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015;16(5):301-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015;195(5):2141-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse.
    Authors: Katano I, Takahashi T, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M
    J Immunol, 2015;194(7):3513-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2013;43(2):480-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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