Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB138G-025
FAB138G-100
Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry.
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Product Details
Citations (9)
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Reviews (2)

Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
Source
Monoclonal Mouse IgG1 Clone # 134591
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of NKG2C/CD159c antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB138G) or (B) Mouse IgG1Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References
  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.
Long Name
Natural Killer G2C
Entrez Gene IDs
3822 (Human); 16642 (Mouse)
Alternate Names
CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Increased Susceptibility of the CD57- NK Cells Expressing KIR2DL2/3 and NKG2C to iCasp9 Gene Retroviral Transduction and the Relationships with Proliferative Potential, Activation Degree, and Death Induction Response
    Authors: AI Palamarchu, NA Alekseeva, MA Streltsova, MO Ustiuzhani, PA Kobyzeva, SA Kust, MV Grechikhin, AA Boyko, OA Shustova, AM Sapozhniko, EI Kovalenko
    International Journal of Molecular Sciences, 2021;22(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. High Frequency Production of T Cell-Derived iPSC Clones Capable of Generating Potent Cytotoxic T Cells
    Authors: S Nagano, T Maeda, H Ichise, S Kashima, M Ohtaka, M Nakanishi, T Kitawaki, N Kadowaki, A Takaori-Ko, K Masuda, H Kawamoto
    Mol Ther Methods Clin Dev, 2020;16(0):126-135.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Systems Vaccinology Identifies an Early Innate Immune Signature as a Correlate of Antibody Responses to the Ebola Vaccine rVSV-ZEBOV
    Authors: A Rechtien, L Richert, H Lorenzo, G Martrus, B Hejblum, C Dahlke, R Kasonta, M Zinser, H Stubbe, U Matschl, A Lohse, V Krähling, M Eickmann, S Becker,, R Thiébaut, M Altfeld, M Addo
    Cell Rep, 2017;20(9):2251-2261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. KIR2DL3 and KIR2DL1 show similar impact on licensing of human NK cells.
    Authors: Sim M, Stowell J, Sergeant R, Altmann D, Long E, Boyton R
    Eur J Immunol, 2016;46(1):185-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015;195(5):2141-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015;16(5):301-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse.
    Authors: Katano I, Takahashi T, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M
    J Immunol, 2015;194(7):3513-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2013;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody

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By Lu Bai on 07/26/2022
Application: Flow Cytometry Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Human NKG2C/CD159c Alexa Fluor® 488-conjugated Antibody
By Anonymous on 08/20/2021
Application: Flow Cytometry Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human