Human NKG2C/CD159c APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB138A-025
FAB138A-100
Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry.
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Product Details
Citations (12)
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Human NKG2C/CD159c APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
Source
Monoclonal Mouse IgG1 Clone # 134591
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of NKG2C/CD159c antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c APC-conjugated Monoclonal Antibody (Catalog # FAB138A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References
  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.
Long Name
Natural Killer G2C
Entrez Gene IDs
3822 (Human); 16642 (Mouse)
Alternate Names
CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor

Product Datasheets

Citations for Human NKG2C/CD159c APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Which NK cell populations mark the high burden of CMV present in all HIV patients beginning ART in Indonesia?
    Authors: IA Ariyanto, R Estiasari, B Karim, IP Wijaya, B Bela, A Soebandrio, P Price, S Lee
    AIDS research and therapy, 2022;19(1):16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Challenging the Conventional Interpretation of HCMV Seronegativity
    Authors: S Waters, S Lee, A Irish, P Price
    Microorganisms, 2021;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Adaptive NKG2C+ natural killer cells are related to exacerbations and nutritional abnormalities in COPD patients
    Authors: S Pascual-Gu, M Ataya, I Ramírez-Ma, J Yélamos, R Chalela, S Bellido, M López-Bote, J Gea
    Respir. Res., 2020;21(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Functional paralysis of human natural killer cells by alphaherpesviruses
    Authors: TM Campbell, BP McSharry, M Steain, TA Russell, DC Tscharke, JJ Kennedy, B Slobedman, A Abendroth
    PLoS Pathog., 2019;15(6):e1007784.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NK cells generate memory-type responses to human cytomegalovirus-infected fibroblasts
    Authors: N Newhook, N Fudge, M Grant
    Eur. J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NKG2C(+)CD57(+) Natural Killer Cell Expansion Parallels Cytomegalovirus-Specific CD8(+) T Cell Evolution towards Senescence
    Authors: John Heath
    J Immunol Res, 2016;2016(0):7470124.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015;112(6):E556-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Impaired NK Cell Responses to Pertussis and H1N1 Influenza Vaccine Antigens in Human Cytomegalovirus-Infected Individuals.
    Authors: Nielsen C, White M, Bottomley C, Lusa C, Rodriguez-Galan A, Turner S, Goodier M, Riley E
    J Immunol, 2015;194(10):4657-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Galectin-9 functionally impairs natural killer cells in humans and mice.
    Authors: Golden-Mason L, McMahan R, Strong M, Reisdorph R, Mahaffey S, Palmer B, Cheng L, Kulesza C, Hirashima M, Niki T, Rosen H
    J Virol, 2013;87(9):4835-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2013;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Plasmodium falciparum-infected erythrocytes induce granzyme B by NK cells through expression of host-Hsp70.
    Authors: Bottger E, Multhoff G, Kun JF, Esen M
    PLoS ONE, 2012;7(3):e33774.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components.
    Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS
    Blood, 2008;111(6):3155-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human NKG2C/CD159c APC-conjugated Antibody
By Stephane Hua on 07/05/2018
Application: Flow Cytometry Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human