Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 134591
View all NKG2C/CD159c Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94

Specificity

Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NKG2C/CD159c APC‑conjugated Antibody

Detection of NKG2C/CD159c antibody in Human PBMC lymphocytes antibody by Flow Cytometry.

Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c APC-conjugated Monoclonal Antibody (Catalog # FAB138A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Cell surface expression of NKG2C and gene expression of CD57+NKG2C+ NK cells in healthy subjects and HIV-1 chronically infected individuals. a Flow cytometric gating strategy of sorted CD57+NKG2C+ NK cells. b Graphical analysis of frequency of CD57+NKG2C+ NK cells in healthy (black) and HIV-1-infected individuals (red). Mann–Whitney test was used to determine significance between frequencies of each group. c Volcano plot displaying upregulated (red), downregulated (blue), and stably expressed (gray) pre-selected genes, involved in NK cell-mediated responses to HIV-1 infection. Size of each data point is calculated as −log10(p-value) × log2(FC), p-value cutoff p < 0.05, fold change cutoff > 2 or <1/2. Transcriptional analysis from six healthy subjects and five HIV-1 chronically infected individuals from one independent experiment is represented Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Cell surface expression of NKG2C and gene expression of CD57+NKG2C+ NK cells in vaccinated individuals. a Flow cytometric gating strategy of sorted CD57+NKG2C+ NK cells. b Graphical analysis of frequency of CD57+NKG2C+ NK cells in vaccinated individual’s pre (blue)- and post (red) vaccination. Wilcoxon matched-pair sign-rank test was used to determine significance between frequencies of each group. c Volcano plot displaying upregulated (red) and stably expressed (gray) pre-selected genes, involved in NK cell-mediated responses to MVA vaccination. Size of each data point is calculated as −log10(p-value) × log2(FC), p-value cutoff p < 0.05, fold change cutoff > 2 or <1/2. Transcriptional analysis from 11 vaccinated individuals and 1 independent experiment is represented Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Distribution of CD57+NKG2C+ NK frequencies in different groups. Flow cytometry was done on PBMC to assess CD57+NKG2C+ NK frequency by gating on lymphocytes, excluding CD3+ cells, gating on CD56dim cells, and plotting NKG2C versus CD57 expression. Horizontal lines bisecting groups represent medians with IQR shown above and below them. Significant differences between medians are shown above lines spanning the groups compared. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27314055), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Frequency of responding NK cells across multiple stimulation conditions. a Top row: overall successive gating strategy demonstrates initial broad gating on forward and side scatter to include large lymphocytes. Forward area and height are used to discriminate single cells followed by identification of viable cells. Monocytes and B cells are excluded using CD14 and CD19, and T cells are excluded using CD3 and CD4. Bottom row: CD56 and CD16 are used to identify NK cells and changes in CD16 expression across stimulation conditions. Black gate is the total NK population and red gate is CD56 dim population. b A univariate analysis of the frequency of CD107a, INF-gamma, TNF, granzyme B, perforin, and eomesodermin expression from the total NK cell population as well as CD57 and CD8 expression are shown. c Pie charts of classic Boolean analysis of five functional markers measured (CD107a, IFN-gamma, TNF, granzyme B, and perforin). Comparison of pies and nonparametric partial permutation tests (Monte Carlo simulation) are performed to determine statistical significance between pies (p-value < 0.0001)69. d Graph of classic Boolean analysis of five functional markers measured. Four healthy subjects from four independent experiments in each stimulation condition are included. CD107a, IFN-gamma, and TNF are mock subtracted (i.e., stimulated condition − unstimulated result). Black bars represent the standard error mean (SEM) for each stimulation condition calculated as the variance of the sampling distribution obtained, equal to the variance of the population divided by the sample size Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human NKG2C/CD159c APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) lymphocytes

Reviewed Applications

Read 1 review rated 5 using FAB138A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Long Name

Natural Killer G2C

Alternate Names

CD159c, KLRC2, NKG2-C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Gene Symbol

KLRC2

Additional NKG2C/CD159c Products

Product Documents for Human NKG2C/CD159c APC‑conjugated Antibody

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Product Specific Notices for Human NKG2C/CD159c APC‑conjugated Antibody

For research use only

Citations for Human NKG2C/CD159c APC‑conjugated Antibody

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  • Human NKG2C/CD159c APC-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 07/05/2018
    Human NKG2C/CD159c APC‑conjugated Antibody FAB138A

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