Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556),
rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa
1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.
Monoclonal Mouse IgG1 Clone # 603532
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3 Ala40-Glu467 Accession # Q9UM47
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Detection of Notch‑3 in BG01V Human Cells by Flow Cytometry
BG01V human embryonic stem cells were stained with Mouse Anti-Human Notch‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1559G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4-CD8- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).
Have you used Human Notch-3 Alexa Fluor® 488-conjugated Antibody?
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