Human Notch-3 Antibody

Catalog # Availability Size / Price Qty
MAB1559
MAB1559-SP
Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry.
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Product Details
Citations (4)
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Human Notch-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 603532
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Notch-3 antibody in BG01V Human Stem Cells antibody by Flow Cytometry. View Larger

Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry. BGO1v human embryonic stem cells were stained with Mouse Anti-Human Notch-3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-3

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4-CD8- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Entrez Gene IDs
4854 (Human); 18131 (Mouse)
Alternate Names
CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3

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Citations for Human Notch-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Therapeutic antibody targeting of Notch3 signaling prevents mural cell loss in CADASIL
    Authors: Machuca-Parra AI, Bigger-Allen AA, Sanchez AV et al.
    J. Exp. Med.
  2. Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model
    Authors: DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström
    Embo Molecular Medicine, 2022-12-16;0(0):e16556.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: ELISA Capture
  3. Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
    Authors: H Kim, B Jang, YJ Kim, JC Choi
    International Journal of Molecular Sciences, 2022-09-11;23(18):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  4. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
    Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A
    J Immunol, 2014-06-09;193(2):950-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC

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