Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 36507
View all NT-4 Primary Antibodies »

Product Specifications

Specificity

Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) BDNF or rhNT-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human NT‑4 Alexa Fluor™ Plus 555‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NT-4

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

References

  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

UniProt

P34130

Additional NT-4 Products

Product Documents for Human NT‑4 Alexa Fluor™ Plus 555‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NT‑4 Alexa Fluor™ Plus 555‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Customer Reviews

There are currently no reviews for this product. Be the first to review Human NT‑4 Alexa Fluor™ Plus 555‑conjugated Antibody and earn rewards!

Have you used Human NT‑4 Alexa Fluor™ Plus 555‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies