Human NT-4 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human NT-4 Antibody Summary

Species Reactivity
Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) BDNF or rhNT-3 is observed.
Monoclonal Mouse IgG1 Clone # 36507
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NT-4
Gly81-Ala210 (predicted)
Accession # P34130
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human NT‑4 (Catalog # 268-N4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-4

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.
Long Name
Neurotrophin 4
Entrez Gene IDs
4909 (Human)
Alternate Names
GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF4; NTF5; NTF5NT5

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Citation for Human NT-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease.
    Authors: Rose JB, Crews L, Rockenstein E, Adame A, Mante M, Hersh LB, Gage FH, Spencer B, Potkar R, Marr RA, Masliah E
    J. Neurosci., 2009;29(4):1115-25.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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