Human NTB-A/SLAMF6 PE-conjugated Antibody

Clone 292811 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB19081P-025
FAB19081P-100
Human NTB-A/SLAMF6 PE-conjugated Antibody in Flow Cytometry
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Product Details
Citations (3)
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Human NTB-A/SLAMF6 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NTB‑A/SLAMF6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BLAME or rhCRACC is observed.
Source
Monoclonal Mouse IgG2A Clone # 292811
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NTB‑A/SLAMF6
Leu28-Lys225
Accession # Q96DU3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of NTB‑A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of NTB‑A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB‑A/SLAMF6 PE‑conjugated Monoclonal Antibody (Catalog # FAB19081P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NTB-A/SLAMF6

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5‑7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

References
  1. Veillette, A. (2006) Immunol. Rev. 214:22.
  2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
  3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
  4. Munitz, A. et al. (2005) J. Immunol. 174:110.
  5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
  6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
  7. Cao, E. et al. (2006) Immunity 25:559.
  8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
  9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
  10. Howie, D. et al. (2005) J. Immunol. 174:5931.
  11. Kumar, K.R. et al. (2006) Science 312:1665.
Long Name
NK-T-B Antigen/SLAM Family Member 6
Entrez Gene IDs
114836 (Human)
Alternate Names
Activating NK receptor; CD352 antigen; CD352; KALIFLJ50657; Ly108; NK-T-B-antigen; NTBA receptor; NTBA; NTB-A; NTB-AMGC104953; NTBAT- and B-cell antigen; SF2000; SLAM family member 6; SLAMF6

Product Datasheets

Citations for Human NTB-A/SLAMF6 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dopamine inhibits the effector functions of activated NK cells via the upregulation of the D5 receptor.
    Authors: Mikulak J, Bozzo L, Roberto A, Pontarini E, Tentorio P, Hudspeth K, Lugli E, Mavilio D
    J Immunol, 2014;193(6):2792-800.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  2. SAP facilitates recruitment and activation of LCK at NTB-A receptors during restimulation-induced cell death.
    Authors: Katz, Gil, Krummey, Scott M, Larsen, Sasha E, Stinson, Jeffrey, Snow, Andrew L
    J Immunol, 2014;192(9):4202-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia.
    Authors: Brunetta E, Fogli M, Varchetta S, Bozzo L, Hudspeth KL, Marcenaro E, Moretta A, Mavilio D
    Blood, 2009;114(18):3822-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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