Human Otx2 Antibody

R&D Systems | Catalog # MAB1979

R&D Systems
Discontinued Product
MAB1979 has been discontinued. View all Otx2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 246826
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Otx2 (isoform b)
Met1-Leu289
Accession # P32243

Specificity

Detects human Otx2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Otx2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Otx2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otx2

Otx2 (orthodenticle 2) is a 289 amino acid protein that is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95(7):3737.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Entrez Gene IDs

5015 (Human)

Gene Symbol

OTX2

UniProt

P32243

Additional Otx2 Products

Product Documents for Human Otx2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Otx2 Antibody

For research use only

Citations for Human Otx2 Antibody

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Protocols

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FAQs

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