Human Otx2 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (5)
Supplemental Products

Human Otx2 Antibody Summary

Species Reactivity
Detects human Otx2 in Western blots.
Monoclonal Mouse IgG2B Clone # 246826
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Otx2 (isoform b)
Accession # P32243
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Otx2
8-25 µg/mL
Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 (orthodenticle 2) is a 289 amino acid protein that is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95(7):3737.
Long Name
Orthodenticle Homolog 2
Entrez Gene IDs
5015 (Human)
Alternate Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

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Citations for Human Otx2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Otx2 promotes granule cell precursor proliferation and Shh-dependent medulloblastoma maintenance in vivo
    Authors: S El Nagar, A Chakroun, C Le Greneur, D Figarella-, T Di Meglio, T Lamonerie, N Billon
    Oncogenesis, 2018;7(8):60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Role of transcription factor Tgif2 in photoreceptor differentiation in the mouse retina
    Exp Eye Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.
    Authors: Williams E, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev G, Mok J, Lin B, Lu J, Chiao C, Cherney R, Li H, Zhang S, Chang Q
    Hum Mol Genet, 2014;23(11):2968-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Genomic amplification of orthodenticle homologue 2 in medulloblastomas.
    Authors: Boon K, Eberhart CG, Riggins GJ
    Cancer Res., 2005;65(3):703-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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