Human Otx2 Antibody

Catalog #: MAB1979
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1979
MAB1979-SP

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Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Otx2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Otx2 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 246826

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Otx2 (isoform b)
Met1-Leu289
Accession # P32243

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Otx2

Immunocytochemistry

8-25 µg/mL

Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 (orthodenticle 2) is a 289 amino acid protein that is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References

Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95(7):3737.

Long Name

Orthodenticle Homolog 2

Entrez Gene IDs

5015 (Human)

Alternate Names

homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Otx2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
International Journal of Molecular Sciences, 2022;23(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Otx2 promotes granule cell precursor proliferation and Shh-dependent medulloblastoma maintenance in vivo
Authors: S El Nagar, A Chakroun, C Le Greneur, D Figarella-, T Di Meglio, T Lamonerie, N Billon
Oncogenesis, 2018;7(8):60.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Role of transcription factor Tgif2 in photoreceptor differentiation in the mouse retina
Exp Eye Res, 2016;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.
Authors: Williams E, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev G, Mok J, Lin B, Lu J, Chiao C, Cherney R, Li H, Zhang S, Chang Q
Hum Mol Genet, 2014;23(11):2968-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Genomic amplification of orthodenticle homologue 2 in medulloblastomas.
Authors: Boon K, Eberhart CG, Riggins GJ
Cancer Res., 2005;65(3):703-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot