Human p38δ Antibody

Discontinued Product

MAB1519 has been discontinued.
View all p38 delta products.
Detection of Human p38δ by Western Blot.
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Human p38δ Antibody Summary

Species Reactivity
Human
Specificity
Detects human p38δ in Western blots. Mouse and rat p38δ reactivity has not been demonstrated but is likely due to their high homology with human p38δ. In Western blots, no cross-reactivity with recombinant p38 alpha, p38 beta, or p38 gamma is observed.
Source
Monoclonal Mouse IgG1 Clone # 197202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p38δ
Met1-Leu365
Accession # O15264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human p38d antibody by Western Blot. View Larger

Detection of Human p38δ by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human p38d Monoclonal Antibody (Catalog # MAB1519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38?, p38d, and p38a (2 ng/lane) were included. A specific band for p38 delta was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 delta

p38δ is also known as p38 mitogen-activated protein kinase (MAPK) delta, MAPK13 and stress-activated protein kinase-4 (SAPK4).

Entrez Gene IDs
5603 (Human)
Alternate Names
EC 2.7.11; EC 2.7.11.24; MAP kinase 13; MAPK 13; MAPK13; MGC99536; mitogen-activated protein kinase 13; Mitogen-activated protein kinase p38 delta; p38 delta; p38delta; PRKM13; SAPK4MAP kinase p38 delta; Stress-activated protein kinase 4

Product Datasheets

FAQs

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