Detects human p38δ in Western blots. Mouse and rat p38δ reactivity has not been demonstrated but is likely due to their high homology with human p38δ. In Western blots, no cross-reactivity with recombinant p38 alpha, p38 beta, or p38 gamma is observed.
Monoclonal Mouse IgG1 Clone # 197202
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human p38δ Met1-Leu365 Accession # O15264
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human p38δ by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human p38δ Monoclonal Antibody (Catalog # MAB1519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38 gamma, p38δ, and p38 alpha (2 ng/lane) were included. A specific band for p38 delta was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 delta
p38δ is also known as p38 mitogen-activated protein kinase (MAPK) delta, MAPK13 and stress-activated protein kinase-4 (SAPK4).
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