Human p38δ Antibody

Catalog #: MAB1519 Datasheet / COA / SDS

Discontinued Product

MAB1519 has been discontinued.

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Detection of Human p38δ by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p38δ Antibody Summary

Species Reactivity

Human

Specificity

Detects human p38δ in Western blots. Mouse and rat p38δ reactivity has not been demonstrated but is likely due to their high homology with human p38δ. In Western blots, no cross-reactivity with recombinant p38 alpha, p38 beta, or p38 gamma is observed.

Source

Monoclonal Mouse IgG₁ Clone # 197202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p38δ
Met1-Leu365
Accession # O15264

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human p38δ by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human p38d Monoclonal Antibody (Catalog # MAB1519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38?, p38d, and p38a (2 ng/lane) were included. A specific band for p38 delta was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 delta

p38δ is also known as p38 mitogen-activated protein kinase (MAPK) delta, MAPK13 and stress-activated protein kinase-4 (SAPK4).

Entrez Gene IDs

5603 (Human)

Alternate Names

EC 2.7.11; EC 2.7.11.24; MAP kinase 13; MAPK 13; MAPK13; MGC99536; mitogen-activated protein kinase 13; Mitogen-activated protein kinase p38 delta; p38 delta; p38delta; PRKM13; SAPK4MAP kinase p38 delta; Stress-activated protein kinase 4