Human PARD3/Par3 Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• APP Processing and Amyloid Plaque Formation
• Cell to Cell Adhesion Disassembly During EMT
• Epithelial Cell Polarity