Human PARD3/Par3 Antibody

Catalog # Availability Size / Price Qty
MAB8030
MAB8030-SP
PARD3/Par3 in HEK293 Human Cell Line.
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Human PARD3/Par3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.
Source
Monoclonal Mouse IgG1 Clone # 833517
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry PARD3/Par3 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

PARD3/Par3 in HEK293 Human Cell Line. PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Long Name
Par-3 Partitioning Defective 3 Homolog
Entrez Gene IDs
56288 (Human); 93742 (Mouse); 81918 (Rat)
Alternate Names
ASIP; Baz; Bazooka; FLJ21015; par-3 partitioning defective 3 homolog (C. elegans); PAR3; PAR3A; PAR3C.elegans) homolog; PARD3; SE2-5L16; SE2-5LT1; SE2-5T2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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