PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 protein binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells. Many tumors overexpress PD-L1 protein making the PD-1:PD-L1 interaction central to several successful cancer immunotherapy approaches.
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Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
R&D Systems | Catalog # DB7H10
Quantikine Quickit version available - results in 90 minutes (QK1146).
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)
Sensitivity
4.52 pg/mL
Assay Range
25-1600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
The Quantikine® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.
Read this White Paper on detecting B7-H1/PD-L1 levels in multiple cancer subtypes.
Read this White Paper on detecting B7-H1/PD-L1 levels in multiple cancer subtypes.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human, Cynomolgus Monkey
Specificity
Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Human Serum (n=36) | 62.5 | 44.5-106 |
| Cynomolgus Monkey Serum (n=10) | 461 | 248-953 |
| Human EDTA plasma (n=36) | 56.2 | 39.1-108 |
| Cynomolgus monkey EDTA plasma (n=10) | 202 | 89.1-497 |
| Human heparin plasma (n=36) | 52.2 | 33.4-110 |
| Sample Type | Mean of Detectable (pg/mL) | Range (pg/mL) |
| Human Urine (n=10) | 34.5 | ND-34.5 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 260 | 458 | 884 | 244 | 466 | 878 |
| Standard Deviation | 4.25 | 6.83 | 14.7 | 15.9 | 25.8 | 66.4 |
| CV% | 1.6 | 1.5 | 1.7 | 6.5 | 5.5 | 7.6 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 257 | 490 | 934 | 249 | 469 | 898 |
| Standard Deviation | 3.75 | 7.01 | 12.7 | 11.1 | 21.7 | 37.7 |
| CV% | 1.5 | 1.4 | 1.4 | 4.5 | 4.6 | 4.2 |
Recovery for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 97-112 |
| DYC002 (1X) (n=1) | 96 | 90-100 |
| EDTA Plasma (n=4) | 90 | 79-100 |
| Heparin Plasma (n=4) | 85 | 76-95 |
| Serum (n=4) | 89 | 81-100 |
| Urine (n=4) | 109 | 100-116 |
Linearity
Scientific Data Images for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve
Human PD-L1/B7-H1 ELISA Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PD-L1/B7-H1
Long Name
Programmed Death Ligand 1
Alternate Names
B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Entrez Gene IDs
Gene Symbol
CD274
Additional PD-L1/B7-H1 Products
Product Documents for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
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Sample Tested: blood plasmaVerified Customer | Posted 11/30/2018
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Sample Tested: Heparin PlasmaVerified Customer | Posted 10/10/2018
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Sample Tested: SerumVerified Customer | Posted 04/04/2018
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Sample Tested: A375 human melanoma cell lineVerified Customer | Posted 08/02/2017
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