Human Phospho-CEP250/CNAP1 (S2421) Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB11339X Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB11339X-100UG

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All CNAP1/CEP250 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-CEP250/CNAP1 (S2421) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Phospho-CEP250/CNAP1 in direct ELISA

Source

Monoclonal Rabbit IgG Clone # 2739C

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human CEP250/CNAP1 S2421 site
Accession # Q9BV73

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CNAP1/CEP250

CEP250 (also known as CNAP1) is a large centrosome-associated protein that plays a crucial role in centrosome duplication and organization during cell division. Centrosomes are organelles play an essential role in cell division, cell polarity, and intracellular transport. In most animal cells, centrosomes consist of a pair of centrioles surrounded by pericentriolar material (PCM).CEP250/CNAP1 is a critical component of the PCM and is required for centrosome duplication and maturation. It forms a bridge between the centrioles and PCM and recruits other PCM components to the centrosome. Phosphorylation of CEP250/CNAP1 may regulate association/dissociation from centrosome. During M phase of mitosis, it is phosphorylated by NEK2, which may trigger the dissociation from the mitotic centrosome. This antibody detects CEP250/CNAP1 phosphorylated at Ser2421.

Long Name

Centrosomal Nek2-Associated Protein 1

Entrez Gene IDs

11190 (Human)

Alternate Names

250-KD; centrosomal Nek2-associated protein 1,250 kDa centrosomal protein; centrosomal protein 250kDa; centrosome associated protein; centrosome-associated protein CEP250; CEP2; CEP250; CNAP1; C-NAP1; MGC88542