Human Phospho-IFN‑ alpha / beta R1 (S535/S539) Antibody
R&D Systems | Catalog # PPS074
Discontinued Product
PPS074 has been discontinued.
View all IFN-alpha/beta R1 products.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S535/S539 of IFN-alpha R1 Subunit I
Specificity
Human IFN-alpha R1, Type I, Subunit I phosphorylated at S535/S539
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-IFN‑ alpha / beta R1 (S535/S539) Antibody
Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot
Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).Applications for Human Phospho-IFN‑ alpha / beta R1 (S535/S539) Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 dilution
Western Blot
1:1000 dilution HEK293 human embryonic kidney cell line transfected with IFN-alpha R1
Note: The molecular weight of the IFN-alpha R1 varies with cell line (different levels of glycosylation) in HEK293 and HeLa cells; the mature form is ~110 - 130 kDa.
Note: The molecular weight of the IFN-alpha R1 varies with cell line (different levels of glycosylation) in HEK293 and HeLa cells; the mature form is ~110 - 130 kDa.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: IFN-alpha/beta R1
References
- Biron, C.A. (2001) Immunity 14:661.
- Kirkwood, J. (2002) Semin. Oncol. 29:18.
- Kumar, K.G. et al. (2003) EMBO J. 22:5480.
Long Name
Interferon alpha/beta Receptor 1
Alternate Names
IFN-aR1, IFN-bR1, IFNAR1, IFNbR1
Gene Symbol
IFNAR1
Additional IFN-alpha/beta R1 Products
Product Documents for Human Phospho-IFN‑ alpha / beta R1 (S535/S539) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Phospho-IFN‑ alpha / beta R1 (S535/S539) Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
