Human Phospho-IFN-alpha / beta  R1 (S535/S539) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Human IFN-alpha R1, Type I, Subunit I phosphorylated at S535/S539
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-S535/S539 of IFN-alpha R1 Subunit I
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution             HEK293 human embryonic kidney cell line transfected with IFN-alpha R1 

    Note:
    The molecular weight of the IFN-alpha R1 varies with cell line (different levels of glycosylation) in HEK293 and HeLa cells; the mature form is ~110 - 130 kDa.
  • Immunohistochemistry
    1:1000 dilution
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot
Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: IFN-alpha/beta R1
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (1,2). These cytokines produce their effects by binding to the Type I IFN Receptor. This receptor contains two subunits; IFNAR1/ alpha 1 and IFNAR2/ alpha 2 Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This downregulation is thought to be influenced by phosphorylation of S535 and S539 in IFN-alpha R1 (3).
  • References:
    1. Biron, C.A. (2001) Immunity 14:661.
    2. Kirkwood, J. (2002) Semin. Oncol. 29:18.
    3. Kumar, K.G. et al. (2003) EMBO J. 22:5480.
  • Long Name:
    Interferon alpha/beta Receptor 1
  • Entrez Gene IDs:
    3454 (Human); 15975 (Mouse)
  • Alternate Names:
    alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1
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