Detection of Phospho-IFN-alpha / beta R1 (S535/S539) by Western Blot
Western blot of immunoprecipitates from HEK293 cells transfected with 1) Mock, 2) IFN-alpha R1 wild type, and 3) IFN-alpha R1 S535A and S539A mutants showing specific immunolabeling of the ~110 kDa to ~130 kDa IFN-alpha R1 wild type. The immunolabeling is absent in the IFN-alpha R1 S535 and S539 mutants (Control). The immunolableing is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: IFN-alpha/beta R1
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (1,2). These cytokines produce their effects by binding to the Type I IFN Receptor. This receptor contains two subunits; IFNAR1/ alpha 1 and IFNAR2/ alpha 2 Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This downregulation is thought to be influenced by phosphorylation of S535 and S539 in IFN-alpha R1 (3).
Biron, C.A. (2001) Immunity 14:661.
Kirkwood, J. (2002) Semin. Oncol. 29:18.
Kumar, K.G. et al. (2003) EMBO J. 22:5480.
Interferon alpha/beta Receptor 1
Entrez Gene IDs:
3454 (Human); 15975 (Mouse)
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1
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