Human Phospho-NFATC1 (S172) Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB5640U Datasheet / COA / SDS

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FAB5640U-100UG

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All NFATC1 Antibodies

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Human Phospho-NFATC1 (S172) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NFATC1 in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 679340

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human S172 site.

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca⁺⁺, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name

Nuclear Factor of Activated T Cells C1

Entrez Gene IDs

4772 (Human); 18018 (Mouse)

Alternate Names

MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1