Human Phospho-NFATC1 (S172) Antibody

Catalog # Availability Size / Price Qty
MAB5640
MAB5640-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human Phospho-NFATC1 (S172) Antibody Summary

Species Reactivity
Human
Specificity
Detects human NFATC1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 679340
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human S172 site.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca++, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name
Nuclear Factor of Activated T Cells C1
Entrez Gene IDs
4772 (Human); 18018 (Mouse)
Alternate Names
MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Product Datasheets

Citation for Human Phospho-NFATC1 (S172) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation
    Authors: J Deng, ES Wang, RW Jenkins, S Li, R Dries, K Yates, S Chhabra, W Huang, H Liu, AR Aref, E Ivanova, CP Paweletz, M Bowden, CW Zhou, GS Herter-Spr, JA Sorrentino, JE Bisi, PH Lizotte, AA Merlino, MM Quinn, LE Bufe, A Yang, Y Zhang, H Zhang, P Gao, T Chen, ME Cavanaugh, AJ Rode, E Haines, PJ Roberts, JC Strum, WG Richards, JH Lorch, S Parangi, V Gunda, GM Boland, R Bueno, S Palakurthi, GJ Freeman, J Ritz, WN Haining, NE Sharpless, H Arthanari, GI Shapiro, DA Barbie, NS Gray, KK Wong
    Cancer Discov, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-NFATC1 (S172) Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human Phospho-NFATC1 (S172) Antibody
By Anonymous on 09/10/2018
Application: WB Sample Tested: HeLa cells,Blood monocytes Species: Human

Human Phospho-NFATC1 (S172) Antibody
By Anonymous on 09/10/2018
Application: ICC/IF Sample Tested: HeLa cells,THP-1 human acute monocytic leukemia cell line Species: Human