Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 679340
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human S172 site.

Specificity

Detects human NFATC1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Phospho-NFATC1 (S172) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Reviewed Applications

Read 4 reviews rated 4 using MAB5640 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca++, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human); 18018 (Mouse)

Gene Symbol

NFATC1

Additional NFATC1 Products

Product Documents for Human Phospho-NFATC1 (S172) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-NFATC1 (S172) Antibody

For research use only

Citations for Human Phospho-NFATC1 (S172) Antibody

Customer Reviews for Human Phospho-NFATC1 (S172) Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Phospho-NFATC1 (S172) Antibody
    Name: Nar Katuwal
    Application: Western Blot
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 04/21/2021
    Human Phospho-NFATC1 (S172) Antibody MAB5640
  • Name: Jignesh Ganeshbhai Patel
    Application: Western Blot
    Sample Tested: HeLa cells and Blood monocytes
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa cells and THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Human Phospho-NFATC1 (S172) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Skeletal muscle
    Species: Mouse
    Verified Customer | Posted 07/19/2017
    Overloaded plantaris muscles have greater calcineurin signalling and less phosphorylated NFATc1 (~120 kDa). **The band at ~80 kDa may represent the dephosphorylated form of NFATc1.
    Human Phospho-NFATC1 (S172) Antibody MAB5640

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