Human Phospho-NFATC1 (S172) Antibody

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Product Details
Citations (3)
Supplemental Products
Reviews (3)

Human Phospho-NFATC1 (S172) Antibody Summary

Species Reactivity
Detects human NFATC1 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 679340
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing the human S172 site.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC1

NFATC1 (Nuclear factor of activated T cells c1), also called NFAT2a, is a 120 kDa, 943 amino acid member of the NFAT family of transcription factors. NFATC1 is found in T cells and mast cells, and regulates cytokine transcription. NFATC1 is normally cytoplasmic and phosphorylated at Ser172 within a transactivation domain. Upon a rise in intracellular Ca++, dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus. Multiple isoforms exist.

Long Name
Nuclear Factor of Activated T Cells C1
Entrez Gene IDs
4772 (Human); 18018 (Mouse)
Alternate Names
MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

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Citations for Human Phospho-NFATC1 (S172) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. GSK3 inhibition with low dose lithium supplementation augments murine muscle fatigue resistance and specific force production
    Authors: Whitley KC, Hamstra SI, Baranowski RW et al.
    Physiol Rep
  2. Neurogranin is expressed in mammalian skeletal muscle and inhibits calcineurin signaling and myoblast fusion
    Authors: Fajardo VA, Watson CJF, Bott KN et al.
    American Journal of Physiology-Cell Physiology
  3. CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation
    Authors: J Deng, ES Wang, RW Jenkins, S Li, R Dries, K Yates, S Chhabra, W Huang, H Liu, AR Aref, E Ivanova, CP Paweletz, M Bowden, CW Zhou, GS Herter-Spr, JA Sorrentino, JE Bisi, PH Lizotte, AA Merlino, MM Quinn, LE Bufe, A Yang, Y Zhang, H Zhang, P Gao, T Chen, ME Cavanaugh, AJ Rode, E Haines, PJ Roberts, JC Strum, WG Richards, JH Lorch, S Parangi, V Gunda, GM Boland, R Bueno, S Palakurthi, GJ Freeman, J Ritz, WN Haining, NE Sharpless, H Arthanari, GI Shapiro, DA Barbie, NS Gray, KK Wong
    Cancer Discov, 2017-11-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Reviews for Human Phospho-NFATC1 (S172) Antibody

Average Rating: 4 (Based on 3 Reviews)

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Human Phospho-NFATC1 (S172) Antibody
By Nar Katuwal on 04/21/2021
Application: WB Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Human Phospho-NFATC1 (S172) Antibody
By Jignesh Ganeshbhai Patel on 09/10/2018
Application: WB Sample Tested: Blood monocytes,HeLa cells Species: Human

Human Phospho-NFATC1 (S172) Antibody
By Jignesh Patel on 09/10/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HeLa cells,THP-1 human acute monocytic leukemia cell line Species: Human