Human Phospho-Paxillin (Y118) Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Product Datasheets

Related Research Areas

• Actin Stress Fibers and Focal Adhesions During EMT
• Adaptor Proteins
• Adaptor Proteins in the Akt Pathway
• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Chemokine Receptor Intracellular Signaling
• Contractile Mechanism and Cytoskeleton in VSMC
• Extracellular Matrix Remodeling and Cell Migration During EMT
• Focal Adhesions
• Integrins and Associated Molecules
• Intracellular Signaling Molecules in Angiogenesis
• Platelet Signaling
• VEGF Family