Human Phospho-Paxillin (Y118) Antibody

Catalog # Availability Size / Price Qty
MAB6164
MAB6164-SP
Detection of Human Phospho-Paxillin (Y118) by Western Blot.
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Human Phospho-Paxillin (Y118) Antibody Summary

Species Reactivity
Human
Specificity
Detects Phospho-Paxillin (Y118) in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 601327
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human Paxillin Y118 site
Accession # P49023
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-Paxillin (Y118) by Western Blot. View Larger

Detection of Human Phospho-Paxillin (Y118) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line
untreated (-) or treated (+) with Pervanadate for 5 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y118) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6164) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Paxillin (Y118) at approximately 80 kDa (as indicated). For additional reference, the membran was stripped and reprobed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF4259). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Entrez Gene IDs
5829 (Human); 19303 (Mouse); 360820 (Rat)
Alternate Names
FLJ16691; Paxillin; PXN

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Secondary Antibodies

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