Human Phospho-Paxillin (Y118) Antibody Summary
Accession # P49023
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Phospho-Paxillin (Y118) by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line
untreated (-) or treated (+) with Pervanadate for 5 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y118) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6164) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Paxillin (Y118) at approximately 80 kDa (as indicated). For additional reference, the membran was stripped and reprobed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF4259). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Phospho-Paxillin (Y118) Antibody
There are currently no reviews for this product. Be the first to review Human Phospho-Paxillin (Y118) Antibody and earn rewards!
Have you used Human Phospho-Paxillin (Y118) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image