Human Phospho-Paxillin (Y118) Antibody

Catalog # Availability Size / Price Qty
Detection of Human Phospho-Paxillin (Y118) by Western Blot.
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Product Details
Citations (2)
Supplemental Products

Human Phospho-Paxillin (Y118) Antibody Summary

Species Reactivity
Detects Phospho-Paxillin (Y118) in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 601327
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing the human Paxillin Y118 site
Accession # P49023
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Paxillin (Y118) antibody by Western Blot. View Larger

Detection of Human Phospho-Paxillin (Y118) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate for 5 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y118) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6164) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Paxillin (Y118) at approximately 80 kDa (as indicated). For additional reference, the membran was stripped and reprobed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (lowerpanel, Catalog # AF4259). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Entrez Gene IDs
5829 (Human); 19303 (Mouse); 360820 (Rat)
Alternate Names
FLJ16691; Paxillin; PXN

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Citations for Human Phospho-Paxillin (Y118) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Identification of lymphocyte cell-specific protein-tyrosine kinase (LCK) as a driver for invasion and migration of oral cancer by tumor heterogeneity exploitation
    Authors: J Wei beta e, J Rosemann, L Müller, M Kappler, AW Eckert, M Gla beta, D Misiak, S Hüttelmaie, WG Ballhausen, M Hatzfeld, M Haemmerle, T Gutschner
    Molecular Cancer, 2021-06-11;20(1):88.
  2. The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK
    Authors: Elianna M. Amin, Yuan Liu, Su Deng, Kay See Tan, Neel Chudgar, Marty W. Mayo et al.
    Science Signaling


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