Human PIAS3 Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PIAS3

PIAS3 (protein inhibitor of activated STAT protein 3) is a 65-75 kDa member of the PIAS family of proteins. It is a ubiquitously expressed nuclear E3-type SUMO ligase that binds to, and inhibits, the gene activating activity of STAT3, MITF and the progesterone receptor. Human PIAS3 is 628 amino acids (aa) in length and contains a SAP domain (aa 11‑45) with an embedded transcriptional coregulator LxxLL motif, a STAT3 binding site (aa 82‑132), a PINIT motif required for nuclear retention (aa 241‑245), an SP-RING-type zinc finger domain (aa 303‑380) and a SUMO1-binding region (aa 450‑460). There is an alternate start site at Met10. Human PIAS3 is 97% aa identical to mouse and canine PIAS3 over both aa 1‑628 and 10‑628.

Product Datasheets

Related Research Areas

• Apoptosis Transcription Factors and Regulators
• Negative Regulators of the Jak/STAT Pathway
• NFkB Pathway
• Stem Cell Transcription Factor Regulators
• Ubiquitin-like Modifiers (UBLs) and Regulators