Western blot shows nuclear extracts of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PIAS3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PIAS3 at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PIAS3 (protein inhibitor of activated STAT protein 3) is a 65-75 kDa member of the PIAS family of proteins. It is a ubiquitously expressed nuclear E3-type SUMO ligase that binds to, and inhibits, the gene activating activity of STAT3, MITF and the progesterone receptor. Human PIAS3 is 628 amino acids (aa) in length and contains a SAP domain (aa 11‑45) with an embedded transcriptional coregulator LxxLL motif, a STAT3 binding site (aa 82‑132), a PINIT motif required for nuclear retention (aa 241‑245), an SP-RING-type zinc finger domain (aa 303‑380) and a SUMO1-binding region (aa 450‑460). There is an alternate start site at Met10. Human PIAS3 is 97% aa identical to mouse and canine PIAS3 over both aa 1‑628 and 10‑628.
Protein Inhibitor of Activated STAT 3
Entrez Gene IDs:
10401 (Human); 229615 (Mouse); 83614 (Rat)
MIZ-type containing 5; PIAS3; Protein inhibitor of activated STAT protein 3; protein inhibitor of activated STAT, 3; ZMIZ5
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