Human PIAS3 Antibody

R&D Systems | Catalog # AF5120

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PIAS3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PIAS3
Met10-Asp628
Accession # Q9Y6X2

Specificity

Detects human PIAS3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PIAS3 Antibody

Detection of Human PIAS3 antibody by Western Blot.

Detection of Human PIAS3 by Western Blot.

Western blot shows nuclear extracts of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PIAS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PIAS3 at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PIAS3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIAS3

PIAS3 (protein inhibitor of activated STAT protein 3) is a 65-75 kDa member of the PIAS family of proteins. It is a ubiquitously expressed nuclear E3-type SUMO ligase that binds to, and inhibits, the gene activating activity of STAT3, MITF and the progesterone receptor. Human PIAS3 is 628 amino acids (aa) in length and contains a SAP domain (aa 11‑45) with an embedded transcriptional coregulator LxxLL motif, a STAT3 binding site (aa 82‑132), a PINIT motif required for nuclear retention (aa 241‑245), an SP-RING-type zinc finger domain (aa 303‑380) and a SUMO1-binding region (aa 450‑460). There is an alternate start site at Met10. Human PIAS3 is 97% aa identical to mouse and canine PIAS3 over both aa 1‑628 and 10‑628.

Long Name

Protein Inhibitor of Activated STAT 3

Alternate Names

ZMIZ5

Entrez Gene IDs

10401 (Human); 229615 (Mouse); 83614 (Rat)

Gene Symbol

PIAS3

UniProt

Q9Y6X2

Additional PIAS3 Products

Product Documents for Human PIAS3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PIAS3 Antibody

For research use only

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