PIAS3 (protein inhibitor of activated STAT protein 3) is a 65-75 kDa member of the PIAS family of proteins. It is a ubiquitously expressed nuclear E3-type SUMO ligase that binds to, and inhibits, the gene activating activity of STAT3, MITF and the progesterone receptor. Human PIAS3 is 628 amino acids (aa) in length and contains a SAP domain (aa 11‑45) with an embedded transcriptional coregulator LxxLL motif, a STAT3 binding site (aa 82‑132), a PINIT motif required for nuclear retention (aa 241‑245), an SP-RING-type zinc finger domain (aa 303‑380) and a SUMO1-binding region (aa 450‑460). There is an alternate start site at Met10. Human PIAS3 is 97% aa identical to mouse and canine PIAS3 over both aa 1‑628 and 10‑628.
Human PIAS3 Alexa Fluor™ Plus 405‑conjugated Antibody
R&D Systems | Catalog # AF5120AFP405
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Specificity
Detects human PIAS3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PIAS3 Alexa Fluor™ Plus 405‑conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PIAS3
Long Name
Protein Inhibitor of Activated STAT 3
Alternate Names
ZMIZ5
Gene Symbol
PIAS3
UniProt
Additional PIAS3 Products
Product Documents for Human PIAS3 Alexa Fluor™ Plus 405‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PIAS3 Alexa Fluor™ Plus 405‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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