Human PKC epsilon Alexa Fluor™ Plus 488‑conjugated Antibody

Name: Human PKC epsilon Alexa Fluor™ Plus 488‑conjugated Antibody
Brand: R&D Systems
SKU: FAB51341AFP488
Price: 509 USD
Availability: InStock

R&D Systems | Catalog # FAB51341AFP488

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG_2B Clone # 666843

View all PKC epsilon Primary Antibodies »

Product Specifications

Specificity

Detects human PKC epsilon in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human PKC epsilon Alexa Fluor™ Plus 488‑conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKC epsilon

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.

Long Name

Protein Kinase C epsilon

Alternate Names

PKCE, PRKCE

Entrez Gene IDs

5581 (Human); 18754 (Mouse); 29340 (Rat)

Gene Symbol

PRKCE

UniProt

Q02156

Additional PKC epsilon Products

Product Documents for Human PKC epsilon Alexa Fluor™ Plus 488‑conjugated Antibody

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PKC epsilon Alexa Fluor™ Plus 488‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only