Human PKC epsilon Antibody

Name: Human PKC epsilon Antibody
Brand: R&D Systems
SKU: MAB51341
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB51341

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 666843

View all PKC epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC epsilon
Gln580-Pro737
Accession # Q02156

Specificity

Detects human PKC epsilon in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human PKC epsilon Antibody

Detection of Human PKC epsilon by Western Blot.

Western blot shows lysates of Human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PKCe Monoclonal Antibody (Catalog # MAB51341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKCe at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PKC epsilon by Simple Western^TM.

Simple Western shows lysates of human hippocampus, loaded at 0.5 mg/ml. A specific band was detected for PKC epsilon at approximately 98 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC epsilon Monoclonal Antibody (Catalog # MAB51341). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human PKC epsilon Antibody

Application

Recommended Usage

Simple Western

5 µg/mL
Sample: Human hippocampus

Western Blot

2 µg/mL
Sample: Human brain (hippocampus) tissue and human brain (hypothalamus) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PKC epsilon

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.