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Human PLA2G1B Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB5018U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5018U-100UG

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Human PLA2G1B Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLA2G1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PLA2G2A, rhPLA2G7, recombinant mouse (rm) PLA2G2A, or rmPLA2R1 is observed.
Source
Monoclonal Mouse IgG2a Clone # 630823
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PLA2G1B
Met1-Ser148
Accession # NM_000928
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PLA2G1B

PLA2G1B, also called Secretory Phopholipase A2, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78% and 76% amino acid sequence identity with mouse and rat PLA2G1B, respectively.

Long Name
Phospholipase A2 Group IB
Entrez Gene IDs
5319 (Human); 18778 (Mouse); 29526 (Rat)
Alternate Names
Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PLA2AMGC119834; PLA2EC 3.1.1.4; PLA2G1B; PPLA2; PPLA2MGC119835

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Product Datasheet
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