Human PLA2G1B Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PLA2G1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PLA2G2A, rhPLA2G7, recombinant mouse (rm) PLA2G2A, or rmPLA2R1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 630823
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PLA2G1B
    Met1-Ser148
    Accession # NM_000928
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human PLA2G1B (Catalog # 5018‑PL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PLA2G1B by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G1B Monoclonal Antibody (Catalog # MAB5018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G1B at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLA2G1B

PLA2G1B, also called Secretory Phopholipase A2, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78% and 76% amino acid sequence identity with mouse and rat PLA2G1B, respectively.

  • Long Name:
    Phospholipase A2 Group IB
  • Entrez Gene IDs:
    5319 (Human); 18778 (Mouse); 29526 (Rat)
  • Alternate Names:
    Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PLA2AMGC119834; PLA2EC 3.1.1.4; PLA2G1B; PPLA2; PPLA2MGC119835
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