PLA2G1B, also called Secretory Phopholipase A2, hydrolyses the sn-2 ester bond of phospholipids, including those in cell membranes, to generate non-esterified free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released into plasma and biological fluids on appropriate cell activation, especially during systemic inflammatory, autoimmune, or allergic disease. PLA2G1B is abundant in digestive organs but is also found elsewhere. Human PLA2G1B shares 78% and 76% amino acid sequence identity with mouse and rat PLA2G1B, respectively.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 630823
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PLA2G1B
Met1-Ser148
Accession # NM_000928
Met1-Ser148
Accession # NM_000928
Specificity
Detects human PLA2G1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PLA2G2A, rhPLA2G7, recombinant mouse (rm) PLA2G2A, or rmPLA2R1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PLA2G1B Antibody
Detection of Human PLA2G1B by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G1B Monoclonal Antibody (Catalog # MAB5018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G1B at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human PLA2G1B Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G1B (Catalog # 5018‑PL)
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G1B (Catalog # 5018‑PL)
Western Blot
2 µg/mL
Sample: Human pancreas tissue under non-reducing conditions only
Sample: Human pancreas tissue under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLA2G1B
Long Name
Phospholipase A2 Group IB
Alternate Names
PLA2, PLA2A, PPLA2
Gene Symbol
PLA2G1B
UniProt
Additional PLA2G1B Products
Product Documents for Human PLA2G1B Antibody
Product Specific Notices for Human PLA2G1B Antibody
For research use only
Related Research Areas
Customer Reviews for Human PLA2G1B Antibody
There are currently no reviews for this product. Be the first to review Human PLA2G1B Antibody and earn rewards!
Have you used Human PLA2G1B Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars